PXD007054

PXD007054 is an original dataset announced via ProteomeXchange.

Title	SUMOylome Profiling in Arabidopsis of Targets Modified by the Sumo Ligase Siz1 During Heat Stress
Description	Ligation is accomplished by a small set of SUMO E3 ligases, with the SAP-MIZ domain-containing (SIZ)-1 and METHYL METHANESULFONATE-SENSITIVE (MMS)-21 ligases having critical roles in stress protection and DNA endoreduplication, respectively. To help identify their corresponding Arabidopsis targets, we combined siz1 and mms21 mutants with proteomic analyses of SUMOylated proteins enriched using an engineered 6His-SUMO1. Through multiple datasets from seedlings grown at normal temperatures or exposed to heat stress, we identified over 1,000 SUMO targets, most of which are nuclear localized. Whereas no targets could be assigned to MMS21, suggesting that it modifies only one or a few low abundance proteins, numerous targets could be assigned to SIZ1, including major transcription factors, co-activators/repressors, and chromatin modifiers connected to abiotic and biotic stress defense, some of which associate into multi-subunit regulatory complexes.
HostingRepository	PRIDE
AnnounceDate	2018-04-05
AnnouncementXML	Submission_2018-04-05_04:10:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Therese Rytz
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-17 01:21:22	ID requested
⏵ 1	2018-04-05 04:10:35	announced

Rytz TC, Miller MJ, McLoughlin F, Augustine RC, Marshall RS, Juan YT, Charng YY, Scalf M, Smith LM, Vierstra RD, SUMOylome Profiling Reveals a Diverse Array of Nuclear Targets Modified by the SUMO Ligase SIZ1 during Heat Stress. Plant Cell, 30(5):1077-1099(2018) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, SUMO, Heat Stress, SIZ1, SUMO E3 Ligases

Richard D Vierstra
contact affiliation	Washington University in St. Louis; University of Wisconsin-Madison
contact email	rdvierstra@wustl.edu
lab head
Therese Rytz
contact affiliation	Washington University in St. Louis
contact email	rytz@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD007054

PRIDE project URI

• PRIDE
  1. PXD007054
     1. Label: PRIDE project
     2. Name: SUMOylome Profiling in Arabidopsis of Targets Modified by the Sumo Ligase Siz1 During Heat Stress