PXD007042

PXD007042 is an original dataset announced via ProteomeXchange.

Title	Identification of Plasmodium yoelii CCR4/CAF1/CAF1d Binding partners and total proteomics of Py17XNL by Immunoprecipitation/nano LC-MS/MS
Description	The transmission of the malaria parasite between mosquitoes and mammals requires translational repression to ensure that only the proper proteins are expressed at the right time, while still allowing the parasite to prepare the mRNAs it will need for the next developmental stage. With relatively few known specific transcription factors (ApiAP2 family) that may specifically initiate gene transcription, Plasmodium parasites also regulate the stability and turnover of transcripts to provide more comprehensive gene regulation. The CAF1/CCR4/NOT complex has been shown in model organisms to be important for not only mRNA degradation, but also translational control through its deadenylases CAF1 and CCR4. However, few proteins that impose translational repression in Plasmodium sexual stages are known, and those that are characterized primarily affect female gametocytes. Therefore, we have characterized two deadenylases and have uncovered their roles in transmission. We have identified and characterized CCR4-1, which we show plays a role in activating male gametocytes, stabilizing transcripts in gametocytes, and regulating host-to-vector transmission. We find that when ccr4-1 is genetically deleted, there is a loss in the coordination of male gametocyte activation and a reduction in the ability of the parasite to productively infect the mosquito, which is independent of its effect upon male activation. Comparative RNA-seq shows that the deletion of ccr4-1 affects many transcripts that are translationally repressed in females, are related to male gamete function, and/or are important for early mosquito stage development. In contrast, we found that genetic deletion of the major deadenylase Caf1 is lethal. However, we observed that expression of only the N-terminal Caf1 domain is permissive, yet prevents proper complex assembly and phenocopies the ccr4-1 deletion. We therefore conclude that the general and transmission-specialized deadenylases of the CAF1/CCR4/NOT complex play critical and intertwined roles in parasite growth and transmission.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:16.390.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Scott Lindner
SpeciesList	scientific name: Plasmodium yoelii yoelii 17XNL; NCBI TaxID: 352914;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-13 13:41:58	ID requested
1	2019-01-07 08:01:20	announced
2	2019-02-07 04:09:18	announced	Updated publication reference for PubMed record(s): 30703164.
⏵ 3	2024-10-22 04:39:17	announced	2024-10-22: Updated project metadata.

Hart KJ, Oberstaller J, Walker MP, Minns AM, Kennedy MF, Padykula I, Adams JH, Lindner SE, Plasmodium male gametocyte development and transmission are critically regulated by the two putative deadenylases of the CAF1/CCR4/NOT complex. PLoS Pathog, 15(1):e1007164(2019) [pubmed]

10.1371/journal.ppat.1007164;

curator keyword: Biological, Biomedical

submitter keyword: CCR4,Plasmodium, PY17XNL, CAF1

Scott Lindner
contact affiliation	Center for Malaria Research, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802
contact email	Scott.Lindner@psu.edu
lab head
Scott Lindner
contact affiliation	Penn State University
contact email	Scott.Lindner@psu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/01/PXD007042

PRIDE project URI

• PRIDE
  1. PXD007042
     1. Label: PRIDE project
     2. Name: Identification of Plasmodium yoelii CCR4/CAF1/CAF1d Binding partners and total proteomics of Py17XNL by Immunoprecipitation/nano LC-MS/MS