PXD007034

PXD007034 is an original dataset announced via ProteomeXchange.

Title	Dynamic T. vaginalis secretome and secretion of β-amalyses
Description	During infection of the urogenital tract, the parasite Trichomonas vaginalis is challenged by factors such as nutrient limitation, the host immune response and coexistence with other members of the microbial fauna. Thus establishment of trichomonad infection depends on multifactorial host-parasite interactions, that involve both contact-dependent mechanisms, such as adherence to vaginal epithelial cells, contact-dependent extracellular killing of host cells, and active phagocytosis of host cells and bacteria, as well as contact independent mechanisms that include secretion of soluble biologically active molecules. To get more insight into the secreted proteome of T. vaginalis we detected and quantified released proteins at consecutive time points. Out of 2072 detected proteins, 89 were estimated to significantly increase in time. These include leishmaniolysin-like metallopeptidases, cathepsin-like cystein peptidases and a number of proteins involved in sugar metabolism, most notably beta-amylases, which may be significant in utilization of host-cell derived glycogen. We further discovered a large group of secreted hypothetical proteins with predicted triple tyrosine domain that may facilitate interaction with the extracellular matrix of the host epithelium.
HostingRepository	PRIDE
AnnounceDate	2017-12-19
AnnouncementXML	Submission_2017-12-19_02:39:59.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Karel Harant
SpeciesList	scientific name: Trichomonas vaginalis; NCBI TaxID: 5722;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-13 00:08:19	ID requested
⏵ 1	2017-12-19 02:40:00	announced

Š, t, á, fkov, á J, Rada P, Meloni D, Ž, á, rsk, ý V, Smutn, á T, Zimmann N, Harant K, Pompach P, Hrd, ý I, Tachezy J, -amylases. Mol Cell Proteomics, 17(2):304-320(2018) [pubmed]

submitter keyword: Trichomonas vaginalis, secretome

Jan Tachezy
contact affiliation	Charles University, Faculty of Science, Department of Parasitology, BIOCEV Průmyslová 595, 252 42 Vestec,
contact email	jan.tachezy@natur.cuni.cz
lab head
Karel Harant
contact affiliation	Charles University
contact email	harant@natur.cuni.cz
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD007034

PRIDE project URI

• PRIDE
  1. PXD007034
     1. Label: PRIDE project
     2. Name: Dynamic T. vaginalis secretome and secretion of β-amalyses