PXD006998

PXD006998 is an original dataset announced via ProteomeXchange.

Title	Human Leukaemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives
Description	Cryopreservation is a routinely used methodology for prolonged storage of viable cells. The use of cryo-protective agents (CPAs) such as dimethylsulfoxide (DMSO), glycerol or trehalose is paramount to reduce cellular cryo-injury but their effectiveness is still limited. The current study focuses on establishing and modulating the proteomic and the corresponding biological profiles associated with the cryo-injury of human leukaemia (HL-60) cells cryopreserved in DMSO alone or DMSO +/- novel CPAs [e.g. nigerose (Nig) or salidroside (Sal)].
HostingRepository	PRIDE
AnnounceDate	2018-11-26
AnnouncementXML	Submission_2018-12-17_04:32:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Juliana Cassoli
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-acetylaminoglucosylated; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-12 04:33:12	ID requested
1	2018-11-26 02:42:41	announced
⏵ 2	2018-12-17 04:32:53	announced	Updated publication reference for PubMed record(s): 30535373.

Al-Otaibi NAS, Cassoli JS, Martins-de-Souza D, Slater NKH, Rahmoune H, Human leukemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives. Gigascience, 8(3):(2019) [pubmed]

curator keyword: Technical

submitter keyword: Cryopreservation, Oxidative stress, Dimethylsulfoxide, Nigerose, Salidroside.

Daniel Martins-de-Souza
contact affiliation	Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas
contact email	dmsouza@unicamp.br
lab head
Juliana Cassoli
contact affiliation	University of Campinas
contact email	jscassoli@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD006998

PRIDE project URI

• PRIDE
  1. PXD006998
     1. Label: PRIDE project
     2. Name: Human Leukaemia cells (HL-60) proteomic and biological signatures underpinning cryo-damage are differentially modulated by novel cryo-additives