PXD006935

PXD006935 is an original dataset announced via ProteomeXchange.

Title	Ciliary motility and axonemal dynein pre-assembly in Drosophila require Wdr92 in association with the HSP90 co-chaperone, R2TP
Description	To clarify the role of Wdr92, and R2TP, in motile cilium formation, we explored its function in Drosophila. We show that Drosophila Wdr92 is a cytoplasmic protein exclusively expressed in motile ciliated cells and is required exclusively for ciliary/flagellar motility. The major effect of its mutation is loss of dynein arms from the axonemes of sensory neuron cilia and sperm flagella. We confirm that Drosophila Wdr92 interacts with R2TP and that R2TP depletion also impairs dynein arm formation. We provide proteomic evidence that Wdr92 is associated especially with heavy chain assembly and propose that it acts as a specificity factor to bring axonemal dynein clients to the R2TP/HSP90 complex. Thus, Wdr92 is a new dynein assembly factor and it strongly reinforces the critical role of HSP90 and co-chaperones in dynein assembly.
HostingRepository	PRIDE
AnnounceDate	2018-04-25
AnnouncementXML	Submission_2018-05-23_00:36:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex von kriegsheim
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-11 05:37:14	ID requested
1	2018-04-25 06:18:14	announced
⏵ 2	2018-05-23 00:36:10	announced	Updated publication reference for PubMed record(s): 29743191.

Zur Lage P, Stefanopoulou P, Styczynska-Soczka K, Quinn N, Mali G, von Kriegsheim A, Mill P, Jarman AP, Ciliary dynein motor preassembly is regulated by Wdr92 in association with HSP90 co-chaperone, R2TP. J Cell Biol, 217(7):2583-2598(2018) [pubmed]

curator keyword: Biological

submitter keyword: Primary Ciliary Dyskinesia

Alex von Kriegsheim
contact affiliation	University of Edinburgh
contact email	alex.vonkriegsheim@igmm.ed.ac.uk
lab head
Alex von kriegsheim
contact affiliation	Systems Biology
contact email	alex.vonkriegsheim@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD006935

PRIDE project URI

• PRIDE
  1. PXD006935
     1. Label: PRIDE project
     2. Name: Ciliary motility and axonemal dynein pre-assembly in Drosophila require Wdr92 in association with the HSP90 co-chaperone, R2TP