PXD006926

PXD006926 is an original dataset announced via ProteomeXchange.

Title	Xenopus A6 cells nanoLC-MS/MS - The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development
Description	The transcriptional events driving specification of the kidney have been well characterized. However, it remains undetermined how initial kidney field size is established, patterned, and proportioned. Lhx1 is a transcription factor expressed in the kidney anlage and is required for specification of the kidney field, but few Lhx1 interacting cofactors or downstream targets have been identified. By tandem-affinity purification, we isolated Furry (FRY), a multifunctional protein that acts as a transcriptional co-repressor of microRNAs. We found that Xenopus embryos depleted of fry exhibit loss of the kidney field, phenocopying the lhx1 depleted animals. In addition, we demonstrated synergism between Fry and Lhx1, identified candidate microRNAs regulated by the pair, and confirmed these microRNA clusters influence specification of the kidney field. Therefore, our data shows that a tissue-specific transcription factor, Lhx1, interacts with a broadly expressed microRNA repressor, Fry, to establish the kidney field.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_11:42:20.195.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006926
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eugenel Espiritu
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355;
ModificationList	Deamidated; Oxidation; Acetyl; Carbamidomethyl; Gln->pyro-Glu
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-11 02:35:10	ID requested
⏵ 1	2021-03-04 11:42:21	announced

Espiritu EB, Crunk AE, Bais A, Hochbaum D, Cervino AS, Phua YL, Butterworth MB, Goto T, Ho J, Hukriede NA, Cirio MC, The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development. Sci Rep, 8(1):16029(2018) [pubmed]

curator keyword: Biological

submitter keyword: Xenopus laevie, A6, kidney epithelial cells, nanoLC-MS/MS

Neil A Hukriede
contact affiliation	Department of Developmental Biology, University of Pittsburgh, USA
contact email	hukriede@pitt.edu
lab head
Eugenel Espiritu
contact affiliation	University of Pittsburgh
contact email	espiritu@pitt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/11/PXD006926

PRIDE project URI

• PRIDE
  1. PXD006926
     1. Label: PRIDE project
     2. Name: Xenopus A6 cells nanoLC-MS/MS - The Lhx1-Ldb1 complex interacts with Furry to regulate microRNA expression during pronephric kidney development