PXD006922

PXD006922 is an original dataset announced via ProteomeXchange.

Title	A multiplex targeted mass spectrometry assay for one-shot flavivirus diagnosis
Description	Targeted proteomic mass spectrometry is emerging as a salient clinical diagnostic tool to track protein biomarkers. Yet, its strong analytical properties have not been exploited in the diagnosis and typing of flavivirus. Here, we report the development of a highly sensitive and specific single-shot robust assay for flavivirus typing and diagnosis using targeted mass spectrometry technology. Our flavivirus parallel reaction monitoring assay (fvPRM) has the ability to track secreted flaviviral non-structural protein 1 (NS1) over a broad diagnostic and typing window with high sensitivity, specificity, extendibility and multiplexing capability. These features, pivotal and pertinent to efficient response towards flavivirus outbreaks, including newly emerging flavivirus strains, and circumvent the limitations of current diagnostic assays. fvPRM thus carries high potential in positioning itself as a forerunner in delivering early and accurate diagnosis for disease management.
HostingRepository	PRIDE
AnnounceDate	2019-03-25
AnnouncementXML	Submission_2019-03-25_08:51:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sheena Wee
SpeciesList	scientific name: Flavivirus; NCBI TaxID: 11051;
ModificationList	6x(13)C: 4x(15)N labeled L-arginine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-10 23:49:40	ID requested
⏵ 1	2019-03-25 08:51:17	announced

Wee S, Alli-Shaik A, Kek R, Swa HLF, Tien WP, Lim VW, Leo YS, Ng LC, Hapuarachchi HC, Gunaratne J, Multiplex targeted mass spectrometry assay for one-shot flavivirus diagnosis. Proc Natl Acad Sci U S A, 116(14):6754-6759(2019) [pubmed]

curator keyword: Biomedical

submitter keyword: Targeted proteomics, Dengue, flavivirus.

Jayantha Gunaratne
contact affiliation	Institute of Molecular and Cell Biology, Agency for Science, Technology and Research, 61 Biopolis Drive, Proteos, Singapore 138673
contact email	jayanthag@imcb.a-star.edu.sg
lab head
Sheena Wee
contact affiliation	IMCB
contact email	swee@imcb.a-star.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/03/PXD006922

PRIDE project URI

• PRIDE
  1. PXD006922
     1. Label: PRIDE project
     2. Name: A multiplex targeted mass spectrometry assay for one-shot flavivirus diagnosis