PXD006914

PXD006914 is an original dataset announced via ProteomeXchange.

Title	Proteomics study of skin tissues from RDEB cSCC, low-risk cSCC and metastasizing cSCC
Description	Cutaneous squamous cell carcinoma (cSCC) is the leading cause of death in recessive dystrophic epidermolysis bullosa (RDEB) patients but remains poorly studied on a proteomic level. We aim to characterize the RDEB-SCC proteome by comparing it to the proteomes of UV-light induced cSCC of non-aggressive and aggressive behavior with which RDEB cSCC share either the well-differentiated morphology or the aggressive behavior, respectively. We applied a direct trypsinization protocol using an acid labile surfactant and label-free quantitation to study formalin-fixed, paraffin-embedded tissues from patients with RDEB cSCC (n=6), low-risk cSCC (n=5) and metastasizing cSCC (n=13) by liquid chromatography tandem-mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2017-11-27
AnnouncementXML	Submission_2017-11-27_02:29:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Christine Föll
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-10 02:44:53	ID requested
⏵ 1	2017-11-27 02:29:42	announced

F, ö, ll MC, Fahrner M, Gretzmeier C, Thoma K, Biniossek ML, Kiritsi D, Meiss F, Schilling O, Nystr, ö, m A, Kern JS, Identification of tissue damage, extracellular matrix remodeling and bacterial challenge as common mechanisms associated with high-risk cutaneous squamous cell carcinomas. Matrix Biol, 66():1-21(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: recessive dystrophic epidermolysis bullosa

cutaneus squamous cell carcinoma

formalin-fixed paraffin-embedded tissue;label-free quantitation

liquid chromatography – tandem mass spectrometry

Oliver Schilling
contact affiliation	Institute of Molecular Medicine and Cell Research University of Freiburg, Stefan Meier Strasse 17, 79104 Freiburg, Germany
contact email	oliver.schilling@mol-med.uni-freiburg.de
lab head
Melanie Christine Föll
contact affiliation	Institute of Molecular Medicine and Cell Research Albert-Ludwigs University, Freiburg
contact email	melanie.foell@mol-med.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD006914

PRIDE project URI

• PRIDE
  1. PXD006914
     1. Label: PRIDE project
     2. Name: Proteomics study of skin tissues from RDEB cSCC, low-risk cSCC and metastasizing cSCC