PXD006910

PXD006910 is an original dataset announced via ProteomeXchange.

Title	Molecular effects of Vitamin C treatment in MSC80 cells, a suitable Schwann cell model
Description	Schwann cells (SCs) are not only decisive to produce the axon-wrapping myelin sheath thus ensuring a proper nerve conduction but also exhibit with trophic function and can direct repair mechanisms of the peripheral nervous system. Consequently, suitable and well-characterized SC in vitro models are needed to perform appropriate pre-clinical studies including the investigation of the complex biochemical adaptations occurring in the peripheral nervous system (PNS) under different (patho)physiological conditions. MSC80 cells represent a SC line derived from mouse used in laboratories as a suitable in vitro system for neuropathological studies. As a comprehensive MSC80 protein catalogue remained elusive, here we introduce the most abundant 9532 proteins identified via mass spectrometry based protein analytics and thus provide the most comprehensive SC protein catalogue published thus far. Hereby, not only proteins causative for inherited neuropathies were covered but it has also been demonstrated that apart from cytoplasmic and nuclear proteins, mitochondrial proteins and such belonging to the protein processing machinery are very well covered. Moreover, we addressed the suitability of MSC80 to examine molecular effect of a drug-treatment by analyzing the proteomic signature of Vitamin C-treated MSC80 cells. Proteomic findings along with further immunological and functional experiments support the concept of a beneficial role influence of Vitamin C on oxidative stress and identified TMX1 as an oxidative stress protective factor in SC which might represent a promising target for therapeutic intervention of PNS disorders with marked oxidative stress burden such as diabetic neuropathy.
HostingRepository	PRIDE
AnnounceDate	2018-08-13
AnnouncementXML	Submission_2018-08-13_09:12:42.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006910
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Vietxuan Phan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Oxidation; Carbamidomethyl; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-10 02:28:13	ID requested
⏵ 1	2018-08-13 09:12:44	announced

Phan V, Schmidt J, Matyash V, Malchow S, Thanisch M, Lorenz C, Diepolder I, Schulz JB, Stenzel W, Roos A, Gess B, ve and Vitamin C-Treated Mouse Schwann Cell Line MSC80: Induction of the Antioxidative Thioredoxin Related Transmembrane Protein 1. J Proteome Res, 17(9):2925-2936(2018) [pubmed]

curator keyword: Biological

submitter keyword: MSC80, Schwann cells, TMX1, protein cataloguing

Andreas Roos
contact affiliation	Dr. rer. nat. Andreas Roos Project Leader "Tissue Proteomics" Leibniz-Institut für Analytische Wissenschaften ISAS e.V.
contact email	Andreas.Roos@newcastle.ac.uk
lab head
Vietxuan Phan
contact affiliation	ISAS
contact email	vietxuan.phan@isas.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006910
     1. Label: PRIDE project
     2. Name: Molecular effects of Vitamin C treatment in MSC80 cells, a suitable Schwann cell model