PXD006905

PXD006905 is an original dataset announced via ProteomeXchange.

Title	Single-cell Proteomics of Developing Cells in Live Vertebrate Embryos by Microprobe High-Resolution Mass Spectrometry
Description	In this work, we developed a microprobe capillary electrophoresis high-resolution mass spectrometry approach to identify proteins in single identified cells in live embryos of the South African clawed frog (Xenopus laevis) and also zebrafish. We used a pulled borosilicate capillary to aspirate a calibrated portion of the cell of interest. Proteins in the collected aspirate were detected and quantified via a bottom-up proteomic workflow that was downscaled to the single cells. The microprobe mass spectrometry platform is scalable to smaller cells in live, morphologically complex, developing embryos. As an example, we used this approach to analyze proteomic changes in cells that form a clone in the early developing Xenopus laevis embryo.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_11:23:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Nemes
SpeciesList	scientific name: Xenopus laevis (African clawed frog); NCBI TaxID: 8355; scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-10 01:18:56	ID requested
⏵ 1	2020-05-26 11:23:15	announced

Lombard-Banek C, Moody SA, Manzini MC, Nemes P, Microsampling Capillary Electrophoresis Mass Spectrometry Enables Single-Cell Proteomics in Complex Tissues: Developing Cell Clones in Live Xenopus laevis and Zebrafish Embryos. Anal Chem, 91(7):4797-4805(2019) [pubmed]

curator keyword: Biological

submitter keyword: Single cell analysis, proteomics, Xenopus, embryo, blastomere, capillary electrophoresis, label free quantification, LFQ, electrospray ionization, mass spectrometry, Orbitrap, Q-Exactive Plus

Peter Nemes
contact affiliation	University of Maryland, College Park
contact email	nemes@umd.edu
lab head
Peter Nemes
contact affiliation	George Washington University
contact email	petern@gwu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD006905

PRIDE project URI

• PRIDE
  1. PXD006905
     1. Label: PRIDE project
     2. Name: Single-cell Proteomics of Developing Cells in Live Vertebrate Embryos by Microprobe High-Resolution Mass Spectrometry