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PXD006904

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-01-11
  • AnnouncementXML: Submission_2018-01-11_02:35:03.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Marc van Oostrum
  • Title: Fc Gamma Receptor (FcγR) Expression and Signaling in the Developing Rat Brain
  • Description: Maternal antibodies specific for antigens in the developing brain are implicated as risk factors for neurodevelopmental disorders, but how these antibodies interfere with neurodevelopment remain speculative. It has been postulated that immunoglobulin G-immune complexes (IgG-IC) activate Fc gamma receptors (FcγR) on non-immune cells in the brain, thereby modulating intracellular signaling and/or internalizing function-blocking antibodies specific for intracellular antigens. However, testing this hypothesis has been hindered by the paucity of data regarding FcγR in the developing brain. Thus, we first investigated FcγR expression in the brain of neonatal male and female rats using quantitative PCR analyses. FcgrIa, FcgrIIa, FcgrIIb, FcgrIIIa and Fcgrt transcripts were detectable in the cortex, hippocampus and cerebellum at postnatal days 1 and 7. These transcripts were also present in primary hippocampal and cortical cell cultures, where their expression was upregulated by IFNγ. In order to confirm protein abundance of FcγRIa, FcγRIIb and FcγRIIIa in cultured hippocampal and cortical neurons and astrocytes on the single cell and tissue level we used immunocytochemistry, western blotting, proteotype analysis, and flow cytometry. The data shows that a subpopulation of these cells co-express the excitatory FcγRIa and the inhibitory FcγRIIb. Functional analysis shows that exposure of hippocampal and cortical cell cultures to IgG-IC increased intracellular calcium and Erk phosphorylation, and triggered FcγR-mediated internalization of IgG. Collectively, these data demonstrate that developing neurons and astrocytes express signaling competent FcγR. which could establish a molecular mode of action of maternal antibodies could influence vulnerability to neurodevelopmental disorders via direct interactions with FcγR on non-immune cells in the developing brain. These findings support the hypothesis that maternal antibodies influence vulnerability to neurodevelopmental disorders via direct interactions with FcγR on non-immune cells in the developing brain.
  • SpeciesList: scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
  • ModificationList: acetylated residue; iodoacetamide derivatized residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-07-10 00:42:52ID requested
12018-01-11 02:35:04announced

Publication List

  1. Stamou M, Grodzki AC, van Oostrum M, Wollscheid B, Lein PJ, Fc gamma receptors are expressed in the developing rat brain and activate downstream signaling molecules upon cross-linking with immune complex. J Neuroinflammation, 15(1):7(2018) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Brain, neuron culture, Fc gamma receptors

Contact List

    Bernd Wollscheid
    • contact affiliation: Department of Health Sciences and Technology (D-HEST) Institute of Molecular Systems Biology (IMSB) ETH Zürich
    • contact email: wbernd@ethz.ch
    • lab head:
    Marc van Oostrum
    • contact affiliation: ETH Zürich
    • contact email: marcv@ethz.ch
    • dataset submitter:

Full Dataset Link List

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