PXD006899

PXD006899 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomics of Desulfotomaculum kuznetsovii grown on methanol and ethanol
Description	The aim of this study was to unravel the methanol metabolism of Desulfotomaculum kuznetsovii. Anaerobic methylotrophs, such as methanogens and acetogens, use a pathway initiated by a cobalamine-containing methanol methyltransferase, whereas aerobic methylotrophs generally oxidize methanol to formaldehyde through a pathway initiated by a methanol dehydrogenase. Sulfate-respiring cells grown with methanol in the presence and absence of cobalt and vitamin B12 were analyzed and compared with cells grown with lactate or ethanol. Proteome analysis showed the presence of two methanol degrading pathways in D. kuznetsovii: a cobalt-dependent methanol methyltransferase and a cobalt-independent alcohol dehydrogenase. This is the first time that two methanol pathways have been shown to be present in a single microorganism, and we hypothesize this can give D. kuznetsovii a competitive advantage.
HostingRepository	PRIDE
AnnounceDate	2018-01-22
AnnouncementXML	Submission_2018-01-22_04:22:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sjef Boeren
SpeciesList	scientific name: Desulfotomaculum kuznetsovii DSM 6115; NCBI TaxID: 760568;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-07 06:16:18	ID requested
⏵ 1	2018-01-22 04:22:02	announced

Sousa DZ, Visser M, van Gelder AH, Boeren S, Pieterse MM, Pinkse MWH, Verhaert PDEM, Vogt C, Franke S, K, ü, mmel S, Stams AJM, The deep-subsurface sulfate reducer Desulfotomaculum kuznetsovii employs two methanol-degrading pathways. Nat Commun, 9(1):239(2018) [pubmed]

curator keyword: Biological

submitter keyword: lactate, ethanol, methanol, metabolism, sulfate reducing bacteria

Sjef Boeren
contact affiliation	Wageningen University
contact email	sjef.boeren@wur.nl
lab head
Sjef Boeren
contact affiliation	Biochemistry
contact email	sjef.boeren@wur.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006899

PRIDE project URI

• PRIDE
  1. PXD006899
     1. Label: PRIDE project
     2. Name: Comparative proteomics of Desulfotomaculum kuznetsovii grown on methanol and ethanol