PXD006890

PXD006890 is an original dataset announced via ProteomeXchange.

Title	Oenothera glazioviana root LC-MS
Description	Proteomic studies were performed to identify the proteins involved in copper (Cu) stress responses of Oenothera glazioviana seedlings. Exposure of 28-d-old seedlings to 50 μM CuSO4 for 3 d led to inhibition of shoot and root growth, and the level of lipid peroxidation in the roots was markedly increased. Compared to shoot, Cu absorbed by Oenothera glazioviana more easily accumulation in root. Label-free proteomic analysis indicated 58 differentially abundance proteins (DAPs) of 3149 total proteins in the roots of O. glazioviana seedlings, of which, 36 were upregulated and 22 were downregulated under Cu stress conditions. Gene Ontology analysis showed that most of the identified proteins could be annotated to signal transduction, detoxification, stress defence, carbohydrate, energy, and protein metabolism, development, and oxidoreduction. We also retrieved 13 proteins from the enriched Kyoto Encyclopaedia of Genes and Genomes and the protein-protein interaction databases related to various pathways, including the citric acid (CA) cycle. Application of exogenous CA to O. glazioviana seedlings exposed to Cu caused alleviation of stress symptoms. Overall, this study provides new understanding into the molecular mechanisms at protein level of Cu response in candidate plants contributed to soil properties.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:49:22.802.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chong Wang
SpeciesList	scientific name: Oenothera glazioviana; NCBI TaxID: 482428;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-07 01:25:11	ID requested
1	2022-02-24 04:57:52	announced
⏵ 2	2023-11-14 08:49:23	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Cu, proteomics,Oenothera glazioviana, LC-MS, differentially abundance proteins

Yahua Chen
contact affiliation	College of Life Sciences, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource, National Joint Local Engineering Research Center for Rural Land Resources Use and Consolidation, Nanjing Agricultural University
contact email	yahuachen@njau.edu.cn
lab head
Chong Wang
contact affiliation	Nanjing Agricultural University
contact email	dacong0623@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD006890

PRIDE project URI

• PRIDE
  1. PXD006890
     1. Label: PRIDE project
     2. Name: Oenothera glazioviana root LC-MS