PXD006881

PXD006881 is an original dataset announced via ProteomeXchange.

Title	Pseudomonas aeruginosa and adhesion
Description	Pseudomonas aeruginosa is a pathogenic micro-organism responsible for many hospital-acquired infections. It is able to adhere to solid surfaces and develop an immobilised community or so-called biofilm. Many studies have been focusing on the use of specific materials to prevent the formation of these biofilms, but the reactivity of the bacteria in contact to surfaces remains unknown. In order to evaluate the impact of different materials on the physiology of Pseudomonas aeruginosa during the first stage of biofilm formation, i.e. adhesion, we investigated the total proteome of cells adhering to three materials: stainless steel, glass and polystyrene. Using tandem mass spectrometry performed at the PAPPSO proteomic platform, 930 proteins were identified, 70 of which were differentially expressed between the materials. Dysregulated proteins belonged to 19 PseudoCAP (Pseudomonas Community Annotation Project) functional classes, with a particular abundance of proteins involved in small molecule transport and membrane proteins. Notably, ten porins or porin precursors were under-produced in bacteria adhering to stainless steel when compared to those adhering to polystyrene and glass. Although adhesion to solid surfaces is an extracellular phenomenon, it involves not only extracellular proteins but also intracellular reactions, as observed with the dysregulation of 11 proteins involved in various metabolisms and five in protein translation. Overall, this work showed that during bacterial adhesion, P. aeruginosa senses the materials concerned and is able to modulate its physiology accordingly.
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-24_11:34:31.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006881
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Morgan Guilbaud
SpeciesList	scientific name: Pseudomonas sp. 'Pseudom. M1'; NCBI TaxID: 427577;
ModificationList	dehydrated residue; deaminated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitap Discovery

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-06 02:24:57	ID requested
⏵ 1	2018-10-24 11:34:32	announced

Guilbaud M, Bruzaud J, Bouffartigues E, Orange N, Guillot A, Aubert-Frambourg A, Monnet V, Herry JM, Chevalier S, Bellon-Fontaine MN, PAO1 Adhering to Solid Surfaces. Front Microbiol, 8():1465(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Pseudomonas aeruginosa, adhesion, abiotic material, proteome modification, porins

Marie-Noëlle BELLON-FONTAINE
contact affiliation	Micalis Institute AgroParisTech INRA 78350 Jouy-en-Josas
contact email	marie-noelle.bellon-fontaine@agroparistech.fr
lab head
Morgan Guilbaud
contact affiliation	AgroParisTech
contact email	morgan.guilbaud@inra.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD006881

PRIDE project URI

• PRIDE
  1. PXD006881
     1. Label: PRIDE project
     2. Name: Pseudomonas aeruginosa and adhesion