PXD006879

PXD006879 is an original dataset announced via ProteomeXchange.

Title	Analysis of the early cellular and humoral responses of Galleria mellonella larvae to infection by Candida albicans
Description	Galleria mellonella larvae were administered an inoculum of Candida albicans and the cellular and proteomic response to infection over the first 24 hours was monitored. The yeast cell density in infected larvae declined initially but replication commenced six hours post-infection. The hemocyte density decreased from 5.2 × 106/ml to 2.5 × 106/ml at 2 hours but increased to 4.2 × 106 at 6 hours but decreased subsequently. Administration of β - glucan to larvae also resulted in an increase in hemocyte density (5.1 ± 0.22 × 106/ml to 6.25 ± 0.25 × 106/ml, p < 0.05) and the population showed an increase in the density of small, granular type cells at 24 hours (p < 0.05). Hemocytes from larvae inoculated with β - glucan showed faster killing of C. albicans cells (53 ± 4.1% (p < 0.01), 64 ± 3.7%, (p < 0.01), respectively) than hemocytes from control larvae (24 ± 11%) at 60 min. Proteomic analysis indicated increased abundance of immune related proteins cecropin-A (5 fold) and prophenoloxidase-activating proteinase-1 (5 fold) 6 hours post infection but by 24 hours there was elevated abundance of muscle (tropomyosin 2 (141 fold), calponin (66 fold), troponin I (62 fold), troponin T (61 fold)) and proteins indicative of cellular stress (glutathione-S-transferase-like protein (114 fold)), fungal dissemination (muscle protein 20-like protein (174 fold)) and tissue breakdown (mitochondrial cytochrome c (10 fold)). Proteins decreased in abundance at 24 hour included β - 1,3 - glucan recognition protein precursor (29 fold), prophenoloxidase subunit 2 (25 fold) and lysozyme-like protein 1 (5 fold). By characterizing the early responses to infection, G. mellonella larvae may be used to model the initial stages of infection processes in mammals.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:39:26.206.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerard Sheehan
SpeciesList	scientific name: Galleria mellonella; NCBI TaxID: 7137;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-07-06 01:55:36	ID requested
1	2017-09-06 06:48:50	announced
⏵ 2	2024-10-22 04:39:30	announced	2024-10-22: Updated project metadata.

Sheehan G, Kavanagh K, Analysis of the early cellular and humoral responses of Galleria mellonella larvae to infection by Candida albicans. Virulence, 9(1):163-172(2018) [pubmed]

curator keyword: Biological

submitter keyword: Candida

Galleria, MS/MS, Infection, Immunity, in vivo screening, quantatitive, Q-exactive, Mini-model

Prof. Kevin Kavanagh
contact affiliation	Department of Biology, Maynooth University, Co. Kildare, Ireland. Phone: + 353 - 1- 708 3859. Fax: + 353 - 1- 708 3845.
contact email	kevin.kavanagh@nuim.ie
lab head
Gerard Sheehan
contact affiliation	Maynooth University
contact email	gerard.sheehan.2013@mumail.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006879

PRIDE project URI

• PRIDE
  1. PXD006879
     1. Label: PRIDE project
     2. Name: Analysis of the early cellular and humoral responses of Galleria mellonella larvae to infection by Candida albicans