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PXD006877

PXD006877 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHaloferax volcanii SILAC study of Oxidative stress
DescriptionSILAC was used to monitor proteome changes to Haloferax volcanii after treatment with oxidizing agent NaOCl. SILAC amino acids used were Lysine(+8) and Arginine(+6). For treatment and control groups 4 replicates were used. In replicates 1 and 3, the control group had supplementation of both light lysine and argninie to the medium and the treatment group was supplemented with both heavy lysine(+8) and arginine(+6). In replicates 2 and 4, the labeling was switched, with the control containing heavy lysine(+8) and arginine(+6) and the treatement was supplemented with light lysine and arginine. After treatment, cells were mixed at a 1:1 ratio (control:treatment) and proteins were extracted and digested with trypsin. For the incorporation test, cells were grown in meduim containing heavy lysine(+8) and argnine(+6) and allowed to grow for 24 hours, then subcultured twice, sequentially after 24 h of growth. To test for incorporation of the heavy amino acids, cells were harvested, proteins extracted, and digested with trypsin.
HostingRepositoryPRIDE
AnnounceDate2018-01-09
AnnouncementXMLSubmission_2018-01-09_03:52:51.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLana McMillan
SpeciesList scientific name: Haloferax volcanii DS2; NCBI TaxID: 309800;
ModificationListmethylthiolated residue; 6x(13)C: 2x(15)N labeled L-lysine; 5x(13)C labeled L-proline; monohydroxylated residue; acetylated residue; 6x(13)C labeled L-arginine
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-07-06 01:17:16ID requested
12018-01-09 03:52:52announced
Publication List
McMillan LJ, Hwang S, Farah RE, Koh J, Chen S, Maupin-Furlow JA, Multiplex quantitative SILAC for analysis of archaeal proteomes: a case study of oxidative stress responses. Environ Microbiol, 20(1):385-401(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Archaea, SILAC, quantitative proteomics, Haloferax volcanii, Oxidative stress, Sodium hypochlorite
Contact List
Julie A. Maupin-Furlow
contact affiliationDepartment of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, Genetics Institute, University of Florida, Gainesville, Florida, 32611
contact emailjmaupin@ufl.edu
lab head
Lana McMillan
contact affiliationUniversity of Florida
contact emaillmcmillan@ufl.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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