PXD006857 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter |
Description | Most E2F-binding sites repress transcription through the recruitment of Retinoblasoma (RB) family members until the end of the G1 cell-cycle phase. Although the MYB promoter contains an E2F-binding site, its transcription is activated shortly after the exit from quiescence, before RB family members inactivation, by unknown mechanisms. We had previously uncovered a nuclear factor distinct from E2F, Myb-sp, whose DNA-binding site overlapped the E2F element and had hypothesized that this factor might overcome the transcriptional repression of MYB by E2F-RB family members. We have purified Myb-sp and discovered that Myc-associated zinc finger proteins (MAZ) are major components. We show that various MAZ isoforms are present in Myb-sp and activate transcription via the MYB-E2F element. Moreover, while forced RB or p130 expression repressed the activity of a luciferase reporter driven by the MYB-E2F element, co-expression of MAZ proteins not only reverted repression, but also activated transcription. Finally, we show that MAZ binds the MYB promoter in vivo, that its binding site is critical for MYB transactivation, and that MAZ knockdown inhibits MYB expression during the exit from quiescence. Together, these data indicate that MAZ is essential to bypass MYB promoter repression by RB family members and to induce MYB expression. |
HostingRepository | PRIDE |
AnnounceDate | 2017-10-05 |
AnnouncementXML | Submission_2017-10-05_00:13:22.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD006857 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Sergio Ciordia |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | autoflex |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-07-03 06:53:40 | ID requested | |
⏵ 1 | 2017-10-05 00:13:23 | announced | |
Publication List
Á, lvaro-Blanco J, Urso K, Chiodo Y, Mart, í, n-Cort, á, zar C, Kourani O, Arco PG, Rodr, í, guez-Mart, í, nez M, Calonge E, Alcam, í J, Redondo JM, Iglesias T, Campanero MR, MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter. Nucleic Acids Res, 45(17):9960-9975(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: maz, myb, quiescence, Retinoblasoma |
Contact List
Miguel R. Campanero |
contact affiliation | Tenured Scientist and Lab Director Instituto de Investigaciones Biomedicas CSIC-UAM |
contact email | mcampanero@iib.uam.es |
lab head | |
Sergio Ciordia |
contact affiliation | National Center for Biotechnology |
contact email | sciordia@cnb.csic.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD006857 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006857
- Label: PRIDE project
- Name: MAZ induces MYB expression during the exit from quiescence via the E2F site in the MYB promoter