PXD006833

PXD006833 is an original dataset announced via ProteomeXchange.

Title	Human bladder,colon,kidney,liver cancer LC MS/MS
Description	We propose that enrichment of low-abundance proteins benefits MPs finding. ProteoMinerTM is an equalizing technique by reducing high-abundance proteins and enriching low-abundance proteins in biological liquids. With triton X-100/TBS buffer extraction, ProteoMinerTM enrichment and peptide fractionation, 25 MPs with 73 unique peptides were identified from four human tissues, including 10 membrane proteins and 6 nucleus proteins. Then 18 MPs were confirmed with at least 2 unique peptide identification and 6 MPs were confirmed with 1 unique peptide identification by PRM assay. Hence these results demonstrated ProteoMinerTM as a powerful means in discovery of MPs.
HostingRepository	PRIDE
AnnounceDate	2017-10-02
AnnouncementXML	Submission_2017-10-02_06:07:33.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006833
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	He Yanbin
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-29 08:03:19	ID requested
⏵ 1	2017-10-02 06:07:34	announced

Li S, He Y, Lin Z, Xu S, Zhou R, Liang F, Wang J, Yang H, Liu S, Ren Y, Digging More Missing Proteins Using an Enrichment Approach with ProteoMiner. J Proteome Res, 16(12):4330-4339(2017) [pubmed]

ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project

curator keyword: Biomedical

submitter keyword: "bladder,colon,kidney,liver,LC MS/MS"

Siqi Liu
contact affiliation	Professor and Director Center of Proteomic Analysis BGI-Shenzhen
contact email	siqiliu@genomics.cn
lab head
He Yanbin
contact affiliation	+86 18565610792
contact email	heyanbin@genomics.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/10/PXD006833

PRIDE project URI

• PRIDE
  1. PXD006833
     1. Label: PRIDE project
     2. Name: Human bladder,colon,kidney,liver cancer LC MS/MS