PXD006829

PXD006829 is an original dataset announced via ProteomeXchange.

Title	BPA Gestational Exposure Affects F1 Capacitated Spermatozoa
Description	Proteomic investigations of spermatozoa provide practical tools for distinguishing normal, functional spermatozoa from abnormal spermatozoa. Indeed, two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry (MS) applies high-throughput industrial applications to identify sperm-specific proteins indicative of chemical exposure. As such, a direct comparison of protein expression profiles between control and exposed cells returned a set of protein markers. Because mature mammalian spermatozoa are virtually incapable of protein synthesis, the predicted protein biomarkers in spermatozoa offer considerable stability for use in clinical application. In the current study, we applied 2-DE coupled with ESI-MS/MS to investigate the modified protein profile in F1 capacitated spermatozoa due to gestational bisphenol-A (BPA) exposure to ascertain whether these proteomic modifications could explain the observed functional alterations in spermatozoa.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:38:22.617.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Md Saidur Rahman
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	micrOTOF-Q III

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-29 02:05:27	ID requested
1	2018-01-02 02:49:38	announced
⏵ 2	2024-10-22 04:38:29	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.7b00668;

Rahman MS, Kwon WS, Ryu DY, Khatun A, Karmakar PC, Ryu BY, Pang MG, Functional and Proteomic Alterations of F1 Capacitated Spermatozoa of Adult Mice Following Gestational Exposure to Bisphenol A. J Proteome Res, 17(1):524-535(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Mouse, Spermatozoa, 2-DE, ESI-MS/MS

Myung-Geol Pang
contact affiliation	Chung-Ang University
contact email	mgpang@cau.ac.kr
lab head
Md Saidur Rahman
contact affiliation	Chung-Ang University
contact email	shohagvet@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006829

PRIDE project URI

• PRIDE
  1. PXD006829
     1. Label: PRIDE project
     2. Name: BPA Gestational Exposure Affects F1 Capacitated Spermatozoa