PXD006820

PXD006820 is an original dataset announced via ProteomeXchange.

Title	Secretome analysis of hypoxia-induced 3T3-L1 adipocytes uncovers novel proteins potentially involved in obesity
Description	In the obese state, as adipose tissue expands, adipocytes become hypoxic and dysfunctional, leading to changes in the pattern of secreted proteins. To better understand the role of hypoxia in the mechanisms linked to obesity, we comparatively analyzed the secretome of differentiated 3T3-L1 adipocytes exposed to normoxia or hypoxia for 24 h. Proteins secreted into the culture media were precipitated using trichloroacetic acid and then digested by trypsin. Peptides were labeled by dimethyl labeling and analyzed by reversed phase nanoscale liquid chromatography coupled to a quadrupole Orbitrap mass spectrometer. Among factors downregulated in hypoxic conditions, we identified Decorin, a member of the leucine-rich proteoglycan family, Tissue Inhibitor of Metalloproteinase-2, Thrombospondin 1 and 2, all multifunctional proteins involved in extracellular matrix (ECM) homeostasis, and angiogenesis. Most findings were confirmed by expression studies of the relative genes in parallel experiments in vitro, in differentiated 3T3-L1 adipocytes, and in vivo, in fat tissues from obese vs. lean rats. Our observations are compatible with the concept that hypoxia may be an early trigger for ECM remodeling and angiogenesis in adipose tissue.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:51.255.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariaconcetta Varano
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	4x(2)H labeled dimethylated L-lysine; monohydroxylated residue; 4x(2)H labeled alpha-dimethylamino N-terminal residue; N6,N6-dimethyl-L-lysine; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-28 01:36:43	ID requested
1	2018-02-27 05:59:49	announced
⏵ 2	2024-10-22 04:42:53	announced	2024-10-22: Updated project metadata.

Laria AE, Messineo S, Arcidiacono B, Varano M, Chiefari E, Semple RK, Rocha N, Russo D, Cuda G, Gaspari M, Brunetti A, Foti DP, Secretome Analysis of Hypoxia-Induced 3T3-L1 Adipocytes Uncovers Novel Proteins Potentially Involved in Obesity. Proteomics, 18(7):e1700260(2018) [pubmed]

10.1002/pmic.201700260;

curator keyword: Biological, Biomedical

submitter keyword: Adipocytes, Obesity, Secretome, Hypoxia, LC-MS/MS

Antonio Brunetti
contact affiliation	Department of Health Sciences, University “Magna Græcia” of Catanzaro, Viale Europa (Località Germaneto), 88100 Catanzaro, Italy
contact email	brunetti@unicz.it
lab head
Mariaconcetta Varano
contact affiliation	University of Catanzaro
contact email	mary.varano1@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD006820

PRIDE project URI

• PRIDE
  1. PXD006820
     1. Label: PRIDE project
     2. Name: Secretome analysis of hypoxia-induced 3T3-L1 adipocytes uncovers novel proteins potentially involved in obesity