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PXD006820

PXD006820 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSecretome analysis of hypoxia-induced 3T3-L1 adipocytes uncovers novel proteins potentially involved in obesity
DescriptionIn the obese state, as adipose tissue expands, adipocytes become hypoxic and dysfunctional, leading to changes in the pattern of secreted proteins. To better understand the role of hypoxia in the mechanisms linked to obesity, we comparatively analyzed the secretome of differentiated 3T3-L1 adipocytes exposed to normoxia or hypoxia for 24 h. Proteins secreted into the culture media were precipitated using trichloroacetic acid and then digested by trypsin. Peptides were labeled by dimethyl labeling and analyzed by reversed phase nanoscale liquid chromatography coupled to a quadrupole Orbitrap mass spectrometer. Among factors downregulated in hypoxic conditions, we identified Decorin, a member of the leucine-rich proteoglycan family, Tissue Inhibitor of Metalloproteinase-2, Thrombospondin 1 and 2, all multifunctional proteins involved in extracellular matrix (ECM) homeostasis, and angiogenesis. Most findings were confirmed by expression studies of the relative genes in parallel experiments in vitro, in differentiated 3T3-L1 adipocytes, and in vivo, in fat tissues from obese vs. lean rats. Our observations are compatible with the concept that hypoxia may be an early trigger for ECM remodeling and angiogenesis in adipose tissue.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:42:51.255.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMariaconcetta Varano
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList4x(2)H labeled dimethylated L-lysine; monohydroxylated residue; 4x(2)H labeled alpha-dimethylamino N-terminal residue; N6,N6-dimethyl-L-lysine; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-06-28 01:36:43ID requested
12018-02-27 05:59:49announced
22024-10-22 04:42:53announced2024-10-22: Updated project metadata.
Publication List
Laria AE, Messineo S, Arcidiacono B, Varano M, Chiefari E, Semple RK, Rocha N, Russo D, Cuda G, Gaspari M, Brunetti A, Foti DP, Secretome Analysis of Hypoxia-Induced 3T3-L1 Adipocytes Uncovers Novel Proteins Potentially Involved in Obesity. Proteomics, 18(7):e1700260(2018) [pubmed]
10.1002/pmic.201700260;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: Adipocytes, Obesity, Secretome, Hypoxia, LC-MS/MS
Contact List
Antonio Brunetti
contact affiliationDepartment of Health Sciences, University “Magna Græcia” of Catanzaro, Viale Europa (Località Germaneto), 88100 Catanzaro, Italy
contact emailbrunetti@unicz.it
lab head
Mariaconcetta Varano
contact affiliationUniversity of Catanzaro
contact emailmary.varano1@gmail.com
dataset submitter
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Dataset FTP location
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PRIDE project URI
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