PXD006817

PXD006817 is an original dataset announced via ProteomeXchange.

Title	Compartmentalization of HP1 proteins in pluripotency acquisition and maintenance
Description	Embryonic stem cells (ESCs) have a hyperdynamic chromatin structure characterized by fewer discrete foci of heterochromatin protein 1 family of proteins (HP1) compared to somatic cells. During reprogramming of somatic cells, depletion of HP1γ early, reduced, while depletion later, enhanced the generation of induced pluripotent stem cells (iPSCs); concomitant with a change from a centromeric to nucleoplasmic localization. To identify the interactome of HP1γ in different biochemical environment in ESCs, we compared protein complexes of HP1γ at 0.42M salt (Dignam et al, 1983), with micrococcal nuclease (MCN) digestion with 0.3M NaCl, and MCN with 0.5M NaCl. To understand the effectors of the change in localization, we isolated protein complexes containing HP1γ in ESCs and compared the profile to that in partially reprogrammed intermediates called pre-iPSCs. Given the differential distribution of the HP1 family in ESCs, we also compared the protein interactome of HP1γ in ESCs with that of HP1α and HP1β. In addition, we probed histone associations of HP1γ in ESCs further by querying the histone post-translational modifications that were detectable. The HP1 proteins themselves are decorated with multiple PTMs, several of which we have found to be novel. Taken together our results reveal the complex contribution of the HP1 proteins to pluripotency.
HostingRepository	PRIDE
AnnounceDate	2017-12-13
AnnouncementXML	Submission_2018-01-24_01:51:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Leah Schaffer
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-27 07:46:45	ID requested
1	2017-12-13 04:41:29	announced
⏵ 2	2018-01-24 01:51:26	announced	Updated publication reference for PubMed record(s): 29358085.

Zaidan NZ, Walker KJ, Brown JE, Schaffer LV, Scalf M, Shortreed MR, Iyer G, Smith LM, Sridharan R, Compartmentalization of HP1 Proteins in Pluripotency Acquisition and Maintenance. Stem Cell Reports, 10(2):627-641(2018) [pubmed]

curator keyword: Biological

submitter keyword: HP1, pluripotency, ESC, iPSC

Rupa Sridharan
contact affiliation	Cell and Regenerative Biology, University of Wisconsin-Madison
contact email	RSridharan@discovery.wisc.edu
lab head
Leah Schaffer
contact affiliation	UW-Madison
contact email	lschaffer2@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD006817

PRIDE project URI

• PRIDE
  1. PXD006817
     1. Label: PRIDE project
     2. Name: Compartmentalization of HP1 proteins in pluripotency acquisition and maintenance