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PXD006814

PXD006814 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleOptimization of quantitative proteomic analysis of clots
DescriptionThe aim of our study was to optimize quantitative proteomic analysis of fibrin clots prepared ex vivo from citrated plasma of the peripheral blood drawn from patients with prior venous thromboembolism. We used a multiple enzyme digestion filter aided sample preparation (MED FASP) method combined with LC-MS/MS analysis performed on a Proxeon Easy-nLC System coupled to Q Exactive HF mass spectrometer. We also evaluated the impact of peptide fractionation with pipet-tip strong anion exchange (SAX) method on the obtained results. Our proteomic approach revealed >500 proteins repeatedly identified in the plasma fibrin clots from patients with venous thromboembolism. The multienzyme digestion (MED) FASP method using three different enzymes: LysC, trypsin and chymotrypsin increased the number of identified peptides and proteins and their sequence coverage as compared to a single and double step digestion. Peptide fractionation with SAX protocol slightly increased the depth of proteomic analyses, but also extended the time needed for sample analysis with LC-MS/MS.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:38:29.133.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJacek Wisniewski
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-06-27 07:12:45ID requested
12017-12-08 00:43:59announced
22024-10-22 04:38:33announced2024-10-22: Updated project metadata.
Publication List
10.1186/s12014-017-9173-x;
Stachowicz A, Siudut J, Suski M, Olszanecki R, Korbut R, Undas A, Wi, ś, niewski JR, Optimization of quantitative proteomic analysis of clots generated from plasma of patients with venous thromboembolism. Clin Proteomics, 14():38(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: proteomics, venous thromboembolism, clot, MED FASP
Contact List
Jacek R Wisniewski
contact affiliationBiochemical Proteomics Group, Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany
contact emailjwisniew@biochem.mpg.de
lab head
Jacek Wisniewski
contact affiliationProteomics
contact emailjwisniew@biochem.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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