PXD006811

PXD006811 is an original dataset announced via ProteomeXchange.

Title	Quantitative chemoproteomic profiling of beta lactones in cancer cells
Description	The spongiolactones are marine natural products with an unusual rearranged spongiane skeleton and a fused β-lactone ring. These compounds have potential anticancer properties, but their mode of action has yet to be explored. Here we employ activity-based protein profiling to identify the targets of a more potent spongiolactone derivative (probe 3) in live cancer cells, and compare these to the target profile of a simpler β-lactone (probe 4). Furthermore, we treat K562 cells with probe 3 and perform quantitative proteomic analysis of the global proteome after 24 hrs. Together, these proteomic data provide the first insights into the covalent mechanism of action of this natural product class.
HostingRepository	PRIDE
AnnounceDate	2023-11-22
AnnouncementXML	Submission_2023-11-22_03:32:14.528.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Megan Wright
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-27 07:07:37	ID requested
⏵ 1	2023-11-22 03:32:14	announced

10.1039/C7CC04990K;

curator keyword: Biomedical

submitter keyword: ABPP, leukemia,chemical proteomics, response to compound

Stephan A Sieber
contact affiliation	Chair of Organic Chemistry II, Technische Universität München, Germany
contact email	stephan.sieber@mytum.de
lab head
Megan Wright
contact affiliation	University of Leeds
contact email	m.h.wright@leeds.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD006811

PRIDE project URI

• PRIDE
  1. PXD006811
     1. Label: PRIDE project
     2. Name: Quantitative chemoproteomic profiling of beta lactones in cancer cells