PXD006809

PXD006809 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics analysis identifies novel candidate substrates of the non-receptor tyrosine kinase, SRMS
Description	SRMS (Src-related kinase lacking C-terminal regulatory tyrosine and N-terminal myristoylation sites) or PTK 70 (Protein tyrosine kinase 70) is a non-receptor tyrosine kinase that belongs to the BRK family kinases (BFKs). The gene encoding SRMS was first discovered in 1994. Yet to date less is known about the cellular role of SRMS primarily due to the unidentified substrates or signaling intermediates regulated by the kinase. In this study, we used phosphotyrosine antibody-based immunoaffinity purification in large-scale label-free quantitative phosphoproteomics to identify novel candidate substrates of SRMS. Our analyses led to the identification of 1259 tyrosine-phosphorylated peptides which mapped to 663 phosphoproteins, exclusively from SRMS-expressing cells. Dok1, a previously characterized SRMS substrate, was also identified in our analyses. Functional enrichment analyses revealed that the candidate SRMS substrates represented various biological processes typically linked to cell growth and RNA metabolism. Analyses of the sequence surrounding the phospho-sites in these proteins led us to identify novel candidate SRMS consensus substrate motifs. We utilized customized high-throughput peptide arrays to validate a subset of the candidate SRMS substrates and motifs identified in our MS-based analyses. Finally, we independently validated Vimentin and Sam68, as bonafide SRMS substrates through in vitro and in vivo assays. Overall, our study led to the identification of a significant number of novel and biologically relevant SRMS candidate substrates, which suggests the involvement of the kinase in a vast array of unexplored cellular functions.
HostingRepository	PRIDE
AnnounceDate	2018-03-09
AnnouncementXML	Submission_2018-03-08_23:55:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Raghuveera Goel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-27 05:57:17	ID requested
1	2018-03-08 06:06:33	announced
2	2018-03-08 07:05:18	announced	Updated project metadata.
⏵ 3	2018-03-08 23:55:58	announced	Updated project metadata.

Goel RK, Paczkowska M, Reimand J, Napper S, Lukong KE, ites (SRMS). Mol Cell Proteomics, 17(5):925-947(2018) [pubmed]

curator keyword: Biological

submitter keyword: SRMS, PTK 70, substrates, BRK, PTK6, FRK, Src, phosphoproteomics, mass spectrometry

Kiven Erique Lukong
contact affiliation	Department of Biochemistry, College of Medicine, University of Saskatchewan
contact email	kel232@mail.usask.ca
lab head
Raghuveera Goel
contact affiliation	University of Saskatchewan
contact email	rkg062@mail.usask.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006809
     1. Label: PRIDE project
     2. Name: Phosphoproteomics analysis identifies novel candidate substrates of the non-receptor tyrosine kinase, SRMS