PXD006807

PXD006807 is an original dataset announced via ProteomeXchange.

Title	Shared and specific targets of Arabidopsis immune MAPKs
Description	In Arabidopsis, mitogen-activated protein kinases MPK3, MPK4 and MPK6 constitute essential relays for a variety of functions including cell division, development and innate immunity. While some substrates of MPK3, MPK4 and MPK6 have been identified, the picture is still far from complete. To identify substrates of these MAPKs in cell division, growth and development we compared the phosphoproteomes of wild-type and mpk3, mpk4 and mpk6. To study the function of these MAPKs in innate immunity, we analyzed their phosphoproteomes following activation by a microbe-associated molecular pattern (MAMP). Partially overlapping substrates were retrieved for all three MAPKs, showing target specificity to one, two or all three MAPKs in different biological processes. More precisely, our results illustrate the fact that the entity to be defined as a specific or a shared substrate for MAPKs is not a phosphoprotein but a particular (S/T)P phosphorylation site in a given protein. As a whole, 152 peptides were identified to be differentially phosphorylated in response to MAMP treatment and/or when compared between genotypes and 70 of them could be classified as putative MAPK targets. Biochemical analysis of a number of putative MAPK substrates by phosphorylation and interaction assays confirmed the global phosphoproteome approach. Our study finally expands the set of MAPK substrates to involve other protein kinases, including calcium-dependent (CDPK) and sugar non-fermenting (SnRK) protein kinases.
HostingRepository	PRIDE
AnnounceDate	2017-12-06
AnnouncementXML	Submission_2017-12-06_07:33:31.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006807
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Delphine PFLIEGER
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-26 23:13:54	ID requested
⏵ 1	2017-12-06 07:33:33	announced

Rayapuram N, Bigeard J, Alhoraibi H, Bonhomme L, Hesse AM, Vinh J, Hirt H, Pflieger D, Mitogen-Activated Protein Kinases (MAPKs) MPK3, MPK4, and MPK6. Mol Cell Proteomics, 17(1):61-80(2018) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis thaliana, MAPKinases, immunity, phosphoproteomics, signaling

Heribert Hirt
contact affiliation	Center for Desert Agriculture, 4700 King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia
contact email	heribert.hirt@kaust.edu.sa
lab head
Delphine PFLIEGER
contact affiliation	CNRS
contact email	delphine.pflieger@cea.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD006807

PRIDE project URI

• PRIDE
  1. PXD006807
     1. Label: PRIDE project
     2. Name: Shared and specific targets of Arabidopsis immune MAPKs