PXD006799

PXD006799 is an original dataset announced via ProteomeXchange.

Title	Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B
Description	The eukaryotic translation initiation factor 5B (eIF5B) is a homolog of the ancient IF2, a translation factor that facilitates initiator methionine-tRNAiMet (met-tRNAiMet) delivery to ribosomes in prokaryotes. IF2 evolved during early anaerobic cellular life and can be traced back to the last universal common ancestor. Here, we show that eIF5B is essential for eukaryotic hypoxia tolerance, reminiscent of how IF2 supports prokaryotic anaerobiosis. Global protein synthesis analyses identified eIF5B as a hypoxia-specific translation factor involved in translocating met-tRNAiMet to initiating ribosomes. Systemic translatome studies revealed that eIF5B-dependent mRNAs encode proteins of central carbon metabolism, fructolysis, and other pathways enhanced in organisms inhabiting hypoxic niches. These pathways rely preferentially on eIF5B rather than eIF2, the canonical initiation factor that delivers met-tRNAiMet during aerobic eukaryotic protein synthesis. We suggest that eIF5B/IF2 was retained during evolution of the aerobic eukaryotic lineage to cope with episodes of oxygen deprivation.
HostingRepository	PRIDE
AnnounceDate	2018-01-03
AnnouncementXML	Submission_2019-02-12_08:31:01.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006799
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jonathan Krieger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Label:13C(6)15N(2); Label:13C(8)15N(2); Cation:Na; Deamidated; Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-26 01:42:11	ID requested
1	2018-01-03 02:39:08	announced
⏵ 2	2019-02-12 08:31:03	announced	Updated publication reference for PubMed record(s): 29298419.

Ho JJD, Balukoff NC, Cervantes G, Malcolm PD, Krieger JR, Lee S, Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B. Cell Rep, 22(1):17-26(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Carbon metabolism, eIF5B, evolution, hypoxia, IF2, MATRIX, oxygen, translation

Stephen Lee
contact affiliation	Department of Biochemistry and Molecular Biology, Miller School of Medicine, University of Miami, Miami, FL 33136, USA 2Sylvester Comprehensive Cancer Center, Miller School of Medicine, University of Miami, Miami, FL 33136, USA
contact email	stephenlee@med.miami.edu
lab head
Jonathan Krieger
contact affiliation	SickKids/University of Toronto
contact email	Jonathan.krieger@sickkids.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006799

PRIDE project URI

• PRIDE
  1. PXD006799
     1. Label: PRIDE project
     2. Name: Oxygen-Sensitive Remodeling of Central Carbon Metabolism by Archaic eIF5B