PXD006796

PXD006796 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Absolute quantification of human milk caseins and the whey/casein ratio during the first year of lactation
Description	Whey proteins and caseins in breast milk provide bioactivities and also have different amino acid composition. Accurate determination of these two major protein classes will therefore provide a better understanding of human milk composition and function and also aid in developing improved infant formulas based on bovine whey proteins and caseins. In this study, we implemented a LC-MS/MS quantitative analysis based on iBAQ label free quantitation to estimate absolute concentrations of alpha-casein, beta-casein and kappa-casein in human milk samples (n=88) collected between day 1 and day 360 post partum. Total protein concentration measured by ninhydrin-based amino acid analysis ranged from 2.03 to 17.52 with a mean of 9.37�3.65 g/L. Casein subunits ranged from 0.04 to 1.68 g/L (alpha-), 0.04 to 4.42 g/L (beta-), and 0.10 to 1.72 g/L (kappa-), with beta-casein having the highest average concentration among the three subunits. Calculated whey/casein ratio ranged from 45:55 to 97:3. Linear regression analyses show significant decreases in total protein, beta-casein, kappa-casein, total casein, and whey/casein ratio during the course of lactation. Our study presents a novel and accurate analysis of human milk casein content, demonstrating a lower casein content than earlier believed, which has implications for improved infants formulas.
HostingRepository	MassIVE
AnnounceDate	2017-11-07
AnnouncementXML	Submission_2017-11-07_10:41:17.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brett Phinney
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Oxidation; Phospho; Deamidated
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-23 16:06:28	ID requested
⏵ 1	2017-11-07 10:41:18	announced

Publication List

Liao Y, Weber D, Xu W, Durbin-Johnson BP, Phinney BS, L, ö, nnerdal B, Absolute Quantification of Human Milk Caseins and the Whey/Casein Ratio during the First Year of Lactation. J Proteome Res, 16(11):4113-4121(2017) [pubmed]

Liao Y, Weber D, Xu W, Durbin-Johnson BP, Phinney BS, L, ö, nnerdal B, Absolute Quantification of Human Milk Caseins and the Whey/Casein Ratio during the First Year of Lactation. J Proteome Res, 16(11):4113-4121(2017) [pubmed]

Keyword List

submitter keyword: milk

submitter keyword: milk

Contact List

Bo L�nnerdal
contact affiliation	UC Davis
contact email	bllonnerdal@ucdavis.edu
lab head
Brett Phinney
contact affiliation	UC Davis
contact email	brettsp@ucdavis.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081195