PXD006791

PXD006791 is an original dataset announced via ProteomeXchange.

Title	The Role of Microtubules in the Trafficking of Ectopic ATP Synthase
Description	Adenosine triphosphate (ATP) synthase is located on the inner membrane of mitochondria which generate ATP for various cellular processes. Our previous studies showed that ATP synthases existed on plasma membrane of several types of cancer cells, which was defined as ectopic ATP synthase. However, the trafficking pathway of ectopic ATP synthase is still unclear. To investigate how ATP synthase subunits are involved in the trafficking process, we performed shot-gun proteomic analysis to reveal the plasma membrane proteins of A549 lung cancer cells and identified four subunits of ATP synthases, ATP5A1, ATP5B, ATP5H, and MT-ATP6. With the results of gene set enrichment analysis, we inferred that ectopic ATP synthase subunits are assembled as complex consistently and then translocated to cell surface through the trafficking of mitochondria. On the other hand, microtubules are involved in many intracellular transport. During protein trafficking, mitochondria and vesicles are transported to their final destination along microtubules by motor proteins such as kinesins and dyneins. To explore the function of microtubules in the trafficking of ectopic ATP synthase, we treated MCF-7 breast and A549 lung cancer cells with nocodazole, a microtubule depolymerization agent, and found that the expression level of ectopic ATP synthase decreased using both immunocytochemistry and flow cytometry. Besides, since kinesin family member 5B (KIF5B) is an important motor proteins involved in the transport of mitochondria, we further performed the immunoprecipitation of KIF5B followed by liquid chromatography−tandem mass spectrometry (LC−MS/MS). The result revealed that one of the mitochondrial outer membrane proteins, dynamin-1 like proteins (Drp1), was able to associate to KIF5B. From our recent research results and other reports show that Drp1 is a mediator of mitochondrial dynamic through regulation of mitochondrial fission. Taken together, our findings suggested that Drp1-KIF5B complex-mediated mitochondrial trafficking along microtubules may play a critical role in the transport of ectopic ATP synthase
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:39.683.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ting-Yu Huang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-23 02:22:00	ID requested
1	2023-05-10 08:51:17	announced
⏵ 2	2023-11-14 08:45:48	announced	2023-11-14: Updated project metadata.

Chang YW, Tony Yang T, Chen MC, Liaw YG, Yin CF, Lin-Yan XQ, Huang TY, Hou JT, Hung YH, Hsu CL, Huang HC, Juan HF, Spatial and temporal dynamics of ATP synthase from mitochondria toward the cell surface. Commun Biol, 6(1):427(2023) [pubmed]

curator keyword: Biological

submitter keyword: Ectopic ATP synthases

proteomic analysis

the trafficking of mitochondria

microtubule

KIF5B

Drp1

Hsueh-Fen Juan
contact affiliation	Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan
contact email	yukijuan@gmail.com
lab head
Ting-Yu Huang
contact affiliation	National Taiwan University
contact email	r04b43013@ntu.edu.tw
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006791
     1. Label: PRIDE project
     2. Name: The Role of Microtubules in the Trafficking of Ectopic ATP Synthase