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PXD006771

PXD006771 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMultiplexed proteomics of a cellular transition from G0 to cell cycle in response to IL-3 activation
DescriptionThere exist similarities and differences in metabolism and physiology between normal proliferative cells and tumor cells. Once a cell enters the cell cycle, metabolic machinery is engaged to facilitate various processes. The kinetics and regulation of these metabolic changes have not been properly evaluated. To correlate the orchestration of these processes with the cell cycle, we analyzed the transition from quiescence to proliferation of a non-malignant murine pro-B lymphocyte cell line in response to IL-3. Using multiplex mass-spectrometry-based proteomics we show that the transition to proliferation shares features generally attributed to cancer cells: up-regulation of glycolysis, lipid metabolism, amino-acid synthesis, and nucleotide synthesis and down-regulation of oxidative phosphorylation and the urea cycle. Furthermore, metabolomic profiling of this transition reveals similarities to cancer-related metabolic pathways. In particular, we find that methionine is consumed at a higher rate than other essential amino acids, with a potential link to maintenance of the epigenome.
HostingRepositoryMassIVE
AnnounceDate2017-06-21
AnnouncementXMLSubmission_2017-06-21_14:26:52.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterHo-Joon Lee
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-06-21 14:03:19ID requested
12017-06-21 14:26:53announced
Publication List
no publication
Keyword List
submitter keyword: TMT, time-course, IL-3, pro-B cells, FL5.12, cell cycle, quiescence, proliferation
Contact List
Marc Kirschner
contact affiliationHarvard Medical School
contact emailmarc@hms.harvard.edu
lab head
Ho-Joon Lee
contact affiliationUniversity of Michigan Medical School
contact emailesavant@med.umich.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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