PXD006771
PXD006771 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Multiplexed proteomics of a cellular transition from G0 to cell cycle in response to IL-3 activation |
Description | There exist similarities and differences in metabolism and physiology between normal proliferative cells and tumor cells. Once a cell enters the cell cycle, metabolic machinery is engaged to facilitate various processes. The kinetics and regulation of these metabolic changes have not been properly evaluated. To correlate the orchestration of these processes with the cell cycle, we analyzed the transition from quiescence to proliferation of a non-malignant murine pro-B lymphocyte cell line in response to IL-3. Using multiplex mass-spectrometry-based proteomics we show that the transition to proliferation shares features generally attributed to cancer cells: up-regulation of glycolysis, lipid metabolism, amino-acid synthesis, and nucleotide synthesis and down-regulation of oxidative phosphorylation and the urea cycle. Furthermore, metabolomic profiling of this transition reveals similarities to cancer-related metabolic pathways. In particular, we find that methionine is consumed at a higher rate than other essential amino acids, with a potential link to maintenance of the epigenome. |
HostingRepository | MassIVE |
AnnounceDate | 2017-06-21 |
AnnouncementXML | Submission_2017-06-21_14:26:52.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ho-Joon Lee |
SpeciesList | scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2017-06-21 14:03:19 | ID requested | |
⏵ 1 | 2017-06-21 14:26:53 | announced |
Publication List
no publication |
Keyword List
submitter keyword: TMT, time-course, IL-3, pro-B cells, FL5.12, cell cycle, quiescence, proliferation |
Contact List
Marc Kirschner | |
---|---|
contact affiliation | Harvard Medical School |
contact email | marc@hms.harvard.edu |
lab head | |
Ho-Joon Lee | |
contact affiliation | University of Michigan Medical School |
contact email | esavant@med.umich.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000081179 |