PXD006770

PXD006770 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	COPD lungs show an attached stratified mucus layer resembling the protective colonic mucus
Description	Mucus accumulation is a key feature of respiratory diseases such as cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD). This is associated with goblet cell metaplasia and mucin overexpression, which can be induced by the increased activity of neutrophil elastase. The aim of the study was to characterization of mucus and epithelial cell proteomics in a porcine pancreatic elastase (PPE) mouse model of COPD/CF. The major proteins detected in mucus plugs obtained from PPE-treated mice included mucins Muc5ac and Muc5b, mucus-related proteins Clca1, Fcgbp, and Bpifb1. These proteins were upregulated in bronchoalveolar lavage (BAL) fluid and epithelial cells in mice exposed to elastase. Similar changes were found in BAL fluid of COPD patients.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:46:08.605.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Liisa Arike
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-21 03:46:09	ID requested
1	2018-10-22 06:55:36	announced
2	2021-09-28 03:22:58	announced	2021-09-28: Updated publication reference for PubMed record(s): 30185674.
⏵ 3	2024-10-22 04:46:17	announced	2024-10-22: Updated project metadata.

Publication List

10.1172/jci.insight.120994;
Fern, á, ndez-Blanco JA, Fakih D, Arike L, Rodr, í, guez-Pi, ñ, eiro AM, Mart, í, nez-Abad B, Skansebo E, Jackson S, Root J, Singh D, McCrae C, Evans CM, Å, strand A, Ermund A, Hansson GC, Attached stratified mucus separates bacteria from the epithelial cells in COPD lungs. JCI Insight, 3(17):(2018) [pubmed]

10.1172/jci.insight.120994;

Fern, á, ndez-Blanco JA, Fakih D, Arike L, Rodr, í, guez-Pi, ñ, eiro AM, Mart, í, nez-Abad B, Skansebo E, Jackson S, Root J, Singh D, McCrae C, Evans CM, Å, strand A, Ermund A, Hansson GC, Attached stratified mucus separates bacteria from the epithelial cells in COPD lungs. JCI Insight, 3(17):(2018) [pubmed]

Keyword List

curator keyword: Biomedical
submitter keyword: COPD, BAL fluid,Mouse, mucins, human, lungs, PPE, quantitative proteomics, plugs, epithelial cells, LC-MS/MS

curator keyword: Biomedical

submitter keyword: COPD, BAL fluid,Mouse, mucins, human, lungs, PPE, quantitative proteomics, plugs, epithelial cells, LC-MS/MS

Contact List

Liisa Arike
contact affiliation	Mucin Biology Group Proteomics Laboratory Department of Medical Biochemistry and Cell Biology University of Gothenburg
contact email	liisaarike@gmail.com
lab head
Liisa Arike
contact affiliation	PhD
contact email	liisaarike@gmail.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD006770
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD006770

PRIDE project URI

Repository Record List

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