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PXD006757

PXD006757 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCEP19-RABL2 GTPase complex in ciliary transport
DescriptionThe mechanisms of how highly conserved intraflagellar transport (IFT) protein complexes direct ciliary entry remain unknown. Exploring human ciliopathy genes, including monogenic obesity syndrome genes, has been a rich source of critical ciliary mechanisms. Using AP-MS purification of interactors of the CEP19 monogenic obesity gene, we identify factors and the first known mechanism for triggering ciliary entry of IFT complexes. CEP19 binds the CEP350 and FOP centriolar components and the highly conserved RABL2 GTPase. We discovered that the CEP19-RABL2 complex is first recruited by the centriolar CEP350/FOP complex, concurrently activates nucleotide exchange to capture and release intraflagellar transport B holocomplexes from a large centriolar pool to intraflagellar transport. The CEP19 network of interacting proteins regulates ciliary entry and ciliation, providing a new example of ciliary mechanisms deficient in monogenic obesity syndromes.
HostingRepositoryPRIDE
AnnounceDate2017-06-21
AnnouncementXMLSubmission_2017-06-21_06:08:38.xml
DigitalObjectIdentifierhttp://dx.doi.org/10.6019/PXD006757
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJanos Demeter
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListPhospho; Propionamide; Glu->pyro-Glu; Deamidated; Methyl; Oxidation; Acetyl; GlyGly; Gln->pyro-Glu
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-06-20 03:35:27ID requested
12017-06-21 06:08:40announced
Publication List
Kanie T, Abbott KL, Mooney NA, Plowey ED, Demeter J, Jackson PK, The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base. Dev Cell, 42(1):22-36.e12(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Cilia, ciliopathy, CEP19-RABL2B complex, Human Genetics, Genetics", obesity, centrosome, centriole, GTPase, proteomics, metabolic syndrome, Bardet-Biedl syndrome, intraflagellar transport, microtubules
Contact List
Peter K. Jackson
contact affiliationBaxter Laboratory for Stem Cell Biology Department of Microbiology & Immunology Stanford University School of Medicine 269 Campus Drive CCSR 3205b Stanford, CA 94305-5175
contact emailpjackson@stanford.edu
lab head
Janos Demeter
contact affiliationStanford University
contact emailjdemeter@stanford.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
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