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PXD006756

PXD006756 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFbxl13 regulates centrosome homeostasis and migration through ubiquitin mediated proteolysis
DescriptionF-box proteins are the variable substrate adaptor subunits of the SCF (Skp1, Cul1, F-box) ubiquitin ligases. The family comprise about 69 members with important roles in cancer pathogenesis and cell proliferation. Among the F-box proteins, Fbxl13 is frequently amplified in several cancer patient cohorts suggesting an important role in promoting cancer pathogenesis. However, the main function of Fbxl13 is unknown and its biochemical mechanism of action remains uncharacterised. Here, we show that Fbxl13 specifically interacts with the centrosomal proteins Centrin-2, Centrin-3, Cep152, and Cep192. Fbxl13 is enriched at the centrosome, where it targets Cep192 for ubiquitin mediated proteolysis. In line with this, Fbxl13 overexpression downregulated centrosomal γ-tubulin, and disrupted centrosomal microtubule arrays. Furthermore, depletion of Fbxl13 in U2OS cells corresponds to defects in centrosome duplication and cell motility. Thus, we propose that Fbxl13 is a novel regulator of centrosome duplication and microtubule nucleation activity, highlighting a potential tumourigenic role of Fbxl13 in promoting cell motility.
HostingRepositoryPRIDE
AnnounceDate2024-10-07
AnnouncementXMLSubmission_2024-10-07_10:08:02.155.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD006756
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPhilip Charles
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Carbamidomethyl
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-06-20 02:57:01ID requested
12024-10-07 10:08:02announced
Publication List
10.15252/EMBR.201744799;
10.6019/PXD006756;
Keyword List
curator keyword: Biological
submitter keyword: Ubiquitin ligase,HEK293T, Fbxl13, Orbitrap Elite, F-box protein
Contact List
Benedikt Kessler
contact affiliationTarget Discovery Institute, Nuffield Department of Medicine, University of Oxford
contact emailbenedikt.kessler@ndm.ox.ac.uk
lab head
Philip Charles
contact affiliationUniversity of Oxford
contact emailphilip.charles@ndm.ox.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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