PXD006756

PXD006756 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Fbxl13 regulates centrosome homeostasis and migration through ubiquitin mediated proteolysis
Description	F-box proteins are the variable substrate adaptor subunits of the SCF (Skp1, Cul1, F-box) ubiquitin ligases. The family comprise about 69 members with important roles in cancer pathogenesis and cell proliferation. Among the F-box proteins, Fbxl13 is frequently amplified in several cancer patient cohorts suggesting an important role in promoting cancer pathogenesis. However, the main function of Fbxl13 is unknown and its biochemical mechanism of action remains uncharacterised. Here, we show that Fbxl13 specifically interacts with the centrosomal proteins Centrin-2, Centrin-3, Cep152, and Cep192. Fbxl13 is enriched at the centrosome, where it targets Cep192 for ubiquitin mediated proteolysis. In line with this, Fbxl13 overexpression downregulated centrosomal γ-tubulin, and disrupted centrosomal microtubule arrays. Furthermore, depletion of Fbxl13 in U2OS cells corresponds to defects in centrosome duplication and cell motility. Thus, we propose that Fbxl13 is a novel regulator of centrosome duplication and microtubule nucleation activity, highlighting a potential tumourigenic role of Fbxl13 in promoting cell motility.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:08:02.155.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006756
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Philip Charles
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-20 02:57:01	ID requested
⏵ 1	2024-10-07 10:08:02	announced

Publication List

10.15252/EMBR.201744799;
10.6019/PXD006756;

10.15252/EMBR.201744799;

10.6019/PXD006756;

Keyword List

curator keyword: Biological
submitter keyword: Ubiquitin ligase,HEK293T, Fbxl13, Orbitrap Elite, F-box protein

curator keyword: Biological

submitter keyword: Ubiquitin ligase,HEK293T, Fbxl13, Orbitrap Elite, F-box protein

Contact List

Benedikt Kessler
contact affiliation	Target Discovery Institute, Nuffield Department of Medicine, University of Oxford
contact email	benedikt.kessler@ndm.ox.ac.uk
lab head
Philip Charles
contact affiliation	University of Oxford
contact email	philip.charles@ndm.ox.ac.uk
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD006756

PRIDE project URI

Repository Record List

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