PXD006736

PXD006736 is an original dataset announced via ProteomeXchange.

Title	Label-free quantification of proteome changes in Chlamydomonas reinhardtii treated with lipid inducing small molecules
Description	A study to investigate the effect of small molecule lipid inducing compounds that leads to hyper-accumulation of lipids in N replete cells of Chlamydomonas reinhardtii. These compounds were identified through a high throughput screening designed for that purpose. The highthrouhput screen (HTS) evaluated 43,783 compounds and identified 367 primary hits. These 367 hits were further retested using a 8-point dilution series (from 0.25 to 30 uM) and verified the activity of 243 compounds that induce the hyper lipid accumulating phenotype in algae. Once the hit compounds were identified and confirmed, we then performed extensive chemoinformatics analysis to look for common scaffolds and identified several common substructures. We then selected 15 top performing compounds from 5 diverse structural groups and tested for biochemical parameters such as growth, lipid accumulating capacity, effect on photosynthetic rates, respiration rates, oxygen consumption rates, analysis of different lipid species to identify and quantify fatty acid species using GC-MS, transcriptome analysis using next generation sequencing (RNASeq), untargeted metabolomics using LC-MS, lipidome analysis using FT-ICR MS. To understand the global changes in the proteome, 2 structurally different compounds were selected and compared to the control without compound treatment.
HostingRepository	PRIDE
AnnounceDate	2017-08-11
AnnouncementXML	Submission_2017-08-11_08:19:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nishikant Wase
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-19 01:15:52	ID requested
⏵ 1	2017-08-11 08:19:12	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Chlamydomonas, biofuels, label free Proteomics, Chemical Biology

Concetta DiRusso
contact affiliation	FATTTLab Department of Biochemistry, 1901 Vine Street, University of Nebraska-Lincoln http://biochem.unl.edu/concetta-c-dirusso
contact email	cdirusso2@unl.edu
lab head
Nishikant Wase
contact affiliation	University of Nebraska Lincoln
contact email	nwase2@unl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD006736

PRIDE project URI

• PRIDE
  1. PXD006736
     1. Label: PRIDE project
     2. Name: Label-free quantification of proteome changes in Chlamydomonas reinhardtii treated with lipid inducing small molecules