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PXD006736

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-08-11
  • AnnouncementXML: Submission_2017-08-11_08:19:12.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Nishikant Wase
  • Title: Label-free quantification of proteome changes in Chlamydomonas reinhardtii treated with lipid inducing small molecules
  • Description: A study to investigate the effect of small molecule lipid inducing compounds that leads to hyper-accumulation of lipids in N replete cells of Chlamydomonas reinhardtii. These compounds were identified through a high throughput screening designed for that purpose. The highthrouhput screen (HTS) evaluated 43,783 compounds and identified 367 primary hits. These 367 hits were further retested using a 8-point dilution series (from 0.25 to 30 uM) and verified the activity of 243 compounds that induce the hyper lipid accumulating phenotype in algae. Once the hit compounds were identified and confirmed, we then performed extensive chemoinformatics analysis to look for common scaffolds and identified several common substructures. We then selected 15 top performing compounds from 5 diverse structural groups and tested for biochemical parameters such as growth, lipid accumulating capacity, effect on photosynthetic rates, respiration rates, oxygen consumption rates, analysis of different lipid species to identify and quantify fatty acid species using GC-MS, transcriptome analysis using next generation sequencing (RNASeq), untargeted metabolomics using LC-MS, lipidome analysis using FT-ICR MS. To understand the global changes in the proteome, 2 structurally different compounds were selected and compared to the control without compound treatment.
  • SpeciesList: scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
  • ModificationList: monohydroxylated residue; iodoacetamide derivatized residue
  • Instrument: Synapt MS

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-06-19 01:15:52ID requested
12017-08-11 08:19:12announced

Publication List

  1. Dataset with its publication pending

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Chlamydomonas, biofuels, label free Proteomics, Chemical Biology

Contact List

    Concetta DiRusso
    • contact affiliation: FATTTLab Department of Biochemistry, 1901 Vine Street, University of Nebraska-Lincoln http://biochem.unl.edu/concetta-c-dirusso
    • contact email: cdirusso2@unl.edu
    • lab head:
    Nishikant Wase
    • contact affiliation: University of Nebraska Lincoln
    • contact email: nwase2@unl.edu
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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