PXD006734

PXD006734 is an original dataset announced via ProteomeXchange.

Title	Thylakoid membranes from Arabidopsis LC-MSMS
Description	The small heat shock protein (sHsp) chaperones are crucial for cell survival and can prevent aggregation of client proteins that partially unfold under destabilizing conditions. Most investigations on the chaperone activity sHsps are based on a limited set of thermosensitive model substrate client proteins since the endogenous targets are often not known. There is a high diversity among sHsps, with a single conserved -sandwich fold domain defining the family, the α-crystallin domain, whereas the N-terminal and C-terminal regions are highly variable in length and sequence among various sHsps and conserved only within orthologues. The endogenous targets are probably also varying among various sHsps, cellular compartments, cell type and organism. Here we have investigated Hsp21, a non-metazoan sHsp expressed in the chloroplasts in green plants, which experience huge environmental fluctuations not least in temperature. We describe how Hsp21 can also interact with the chloroplast thylakoid membranes, both when isolated thylakoid membranes are incubated with Hsp21 protein and when plants are heat-stressed. The amount of Hsp21 associated with the thylakoid membranes was precisely determined by quantitative mass spectrometry after metabolic 15N-isotope labelling, of either recombinantly expressed and purified Hsp21 protein or intact Arabidopsis thaliana plants. We found that Hsp21 is among few proteins that become associated with the thylakoid membranes in heat-stressed plants, and that approximately two thirds of the pool of chloroplast Hsp21 is affected. We conclude that for a complete picture of the role of sHsps in plant stress resistance also their association with the membranes should be considered
HostingRepository	PRIDE
AnnounceDate	2017-06-21
AnnouncementXML	Submission_2017-06-21_06:01:36.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gudrun Rutsdottir
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-16 07:56:19	ID requested
⏵ 1	2017-06-21 06:01:37	announced

Bernfur K, Rutsdottir G, Emanuelsson C, The chloroplast-localized small heat shock protein Hsp21 associates with the thylakoid membranes in heat-stressed plants. Protein Sci, 26(9):1773-1784(2017) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis, thylakoid, Hsp21, stable isotope labelling, chaperone, heat shock protein, membranes, photosynthesis, quantitative mass spectrometry, stress response, thermomemory

Cecilia Emanuelsson
contact affiliation	Deparment of Boichemistry and Structural Biology Center for Molecular Protein Science Lund University PO Box 124 22100 Lund Sweden
contact email	cecilia.emanuelsson@biochemistry.lu.se
lab head
Gudrun Rutsdottir
contact affiliation	Department of Biochemistry and Structural Biology Kemicentrum Box 118, 221 00 LUND Sweden
contact email	gvudrun@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD006734

PRIDE project URI

• PRIDE
  1. PXD006734
     1. Label: PRIDE project
     2. Name: Thylakoid membranes from Arabidopsis LC-MSMS