PXD006726

PXD006726 is an original dataset announced via ProteomeXchange.

Title	OMA-1, LIN-41 IPMS
Description	An extended meiotic prophase is a hallmark of oogenesis. Hormonal signaling activates the CDK1/cyclin B kinase to promote oocyte meiotic maturation, which involves nuclear and cytoplasmic events. Nuclear maturation encompasses nuclear envelope breakdown, meiotic spindle assembly, and chromosome segregation. Cytoplasmic maturation involves major changes in oocyte protein translation and cytoplasmic organelles and is poorly understood. In the nematode Caenorhabditis elegans, sperm release the major sperm protein (MSP) hormone to promote oocyte growth and meiotic maturation. Large translational regulatory ribonucleoprotein (RNP) complexes containing the RNA-binding proteins OMA-1, OMA-2, and LIN-41 regulate meiotic maturation downstream of MSP signaling. To understand the control of translation during meiotic maturation, we purified LIN-41-containing RNPs and characterized their protein and RNA components. Protein constituents of LIN-41 RNPs include essential RNA-binding proteins, the GLD-2 cytoplasmic poly(A) polymerase, the CCR4-NOT deadenylase complex, and translation initiation factors. RNA sequencing defined mRNAs associated with both LIN-41 and OMA-1, as well as sets of mRNAs associated with either LIN-41 or OMA-1. Genetic and genomic evidence suggests that GLD-2, which is a component of LIN-41 RNPs, stimulates the efficient translation of many LIN-41-associated transcripts. We analyzed the translational regulation of two transcripts specifically associated with LIN-41 that encode the RNA regulators SPN-4 and MEG-1. We found that LIN-41 represses translation of spn-4 and meg-1, whereas OMA-1 and OMA-2 promote their expression. Upon their synthesis, SPN-4 and MEG-1 assemble into LIN-41 RNPs prior to their functions in the embryo. This study defines a translational repression-to-activation switch as a key element of cytoplasmic maturation.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_04:55:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	micah gearhart
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-15 03:02:43	ID requested
1	2017-06-16 10:30:32	announced
2	2017-06-20 05:45:02	announced	Updated project metadata.
⏵ 3	2017-10-24 04:55:21	announced	Updated project metadata.

Tsukamoto T, Gearhart MD, Spike CA, Huelgas-Morales G, Mews M, Boag PR, Beilharz TH, Greenstein D, . Genetics, 206(4):2007-2039(2017) [pubmed]

curator keyword: Biological

submitter keyword: OMA-1 LIN-41 ribonucleoprotein complex

David Greenstein
contact affiliation	Department of Genetics, Cell Biology, and Development, University of Minnesota, 4-208 MCB, 420 Washington Avenue SE, Minneapolis, MN 55455. Tel: 612-624-3955; FAX: 612-626-6140.
contact email	green959@umn.edu
lab head
micah gearhart
contact affiliation	University of Minnesota
contact email	gearh006@umn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD006726

PRIDE project URI

• PRIDE
  1. PXD006726
     1. Label: PRIDE project
     2. Name: OMA-1, LIN-41 IPMS