Updated publication reference for PubMed record(s): 29608554. These data cover all of the analyses described in the paper "Specter: linear deconvolution as a new paradigm for targeted analysis of data-independent acquisition mass spectrometry proteomics". Specifically, the data consist of - 20 DIA and DDA files from the HEK293T/synthetic phosphopeptides spike-in experiments - 10 DDA files, one for each of ten fractions of an E. coli lysate digest - 14 DIA files for the experiments involving mixtures of synthetic peptides - 11 DDA files for the isolated runs of these synthetic peptides - 84 DIA files for measurements of the phosphoproteome of perturbed PC3 cells - 10 DDA files for spectral library construction for the phosphoproteomics data - 3 DIA and 3 DDA files for analysis of an unfractionated E. coli lysate digest. See the spreadsheet "Specter Datasets Catalog.xlsx" for further descriptions and file metadata.