PXD006701

PXD006701 is an original dataset announced via ProteomeXchange.

Title	The role of HIF1 in enhancing radio resistance of mouse MSCs
Description	Mesenchymal stromal cells (MSCs) are multipotent progenitors supporting bone marrow hematopoiesis. MSC have an efficient DNA damage response (DDR) and are consequently reatively radio-resistant cells. Therefore, MSCs are key to hematopoietic reconstitution following total body irradiation (TBI) and bone marrow transplantation (BMT). The bone marrow niche is hypoxic and via the heterodimeric transcription factor Hypoxia-inducible factor-1 (Hif-1), hypoxia enhances the DDR. Using gene knock-down, we have previously shown that the Hif-1α subunit of Hif is involved in MSC radio-resistance, however its exact mechanism of action remains unknown. In order to dissect the involvement of Hif-1α in the DDR, we have generated using CRISPR/Cas9 technology, a stable MS5 mouse MSC cell line lacking Hif-1 expression. Herein, we show that it is the whole Hif-1 transcription factor, and not only the Hif-1α subunit, that modulates the DDR of mouse MSCs, and that this effect is dependent upon the integrity of the DNA binding domain. We have also characterized the Hif-1α-dependent proteomic changes undergone by hypoxic MS5 cells. These findings have important implications for the modulation of MSC radio-resistance in the context of BMT and cancer.
HostingRepository	PRIDE
AnnounceDate	2018-05-16
AnnouncementXML	Submission_2018-05-16_02:01:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eugene Dillon
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; iodoacetic acid derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-12 07:52:57	ID requested
⏵ 1	2018-05-16 02:01:07	announced

Calvo-Asensio I, Dillon ET, Lowndes NF, Ceredig R, The Transcription Factor Hif-1 Enhances the Radio-Resistance of Mouse MSCs. Front Physiol, 9():439(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Mesenchymal stromal cells, DNA damage response, ionizing radiation, hypoxia, label-free proteomics

Rhodri Ceredig
contact affiliation	Rhodri Ceredig, Professor of Immunology, Discipline of Physiology, College of Medicine & Nursing Health Science, Room 217 Orbsen Building National University of Ireland, Galway H91 DK59
contact email	rhodri.ceredig@nuigalway.ie
lab head
Eugene Dillon
contact affiliation	UCD
contact email	eugene.dillon@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD006701

PRIDE project URI

• PRIDE
  1. PXD006701
     1. Label: PRIDE project
     2. Name: The role of HIF1 in enhancing radio resistance of mouse MSCs