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PXD006700

PXD006700 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTwo-Step Co-Immunoprecipitation (TIP) Enables an Efficient and Highly Selective Isolation of Native Protein-Complexes
DescriptionCo-immunoprecipitation (co-IP) is the most frequently used technique for the isolation of protein-protein (PPI) or protein-nucleic acid interactions (PNI) under native expression conditions. A major disadvantage of co-IP is the abundance of non-specific binders that can impede downstream applications. To overcome this limitation, we developed the two-step co-IP (TIP) that significantly improves the purification of native protein-containing complexes. TIP can be applied with a broad range of specific mono- and polyclonal antibodies. Using TIP we purified IKKalpha- and CD95- interacting proteins in primary human T cells, detected all major binders by mass spectrometry analysis and identified two novel CD95-associated proteins.
HostingRepositoryPRIDE
AnnounceDate2018-01-11
AnnouncementXMLSubmission_2018-01-11_02:57:16.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUwe Warnken
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-06-12 06:09:52ID requested
12018-01-11 02:57:17announced
Publication List
Sciuto MR, Warnken U, Schn, รถ, lzer M, Valvo C, Brunetto L, Boe A, Biffoni M, Krammer PH, De Maria R, Haas TL, Two-Step Coimmunoprecipitation (TIP) Enables Efficient and Highly Selective Isolation of Native Protein Complexes. Mol Cell Proteomics, 17(5):993-1009(2018) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: Immunoprecipitation, co-immunoprecipitation, co-IP, TIP, bTIP, IKK, NFKB, CD95, CHIP, monoclonal, polyclonal, bridging, antibody, chromatin, primary cells
Contact List
Martina Schnoelzer
contact affiliationGerman Cancer Research Center (DKFZ), Heidelberg, Germany Functional Proteome Analysis
contact emailm.schnoelzer@dkfz.de
lab head
Uwe Warnken
contact affiliationGerman Cancer Research Center (DKFZ)
contact emailU.Warnken@dkfz.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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