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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-01-11
  • AnnouncementXML: Submission_2018-01-11_02:57:16.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Uwe Warnken
  • Title: Two-Step Co-Immunoprecipitation (TIP) Enables an Efficient and Highly Selective Isolation of Native Protein-Complexes
  • Description: Co-immunoprecipitation (co-IP) is the most frequently used technique for the isolation of protein-protein (PPI) or protein-nucleic acid interactions (PNI) under native expression conditions. A major disadvantage of co-IP is the abundance of non-specific binders that can impede downstream applications. To overcome this limitation, we developed the two-step co-IP (TIP) that significantly improves the purification of native protein-containing complexes. TIP can be applied with a broad range of specific mono- and polyclonal antibodies. Using TIP we purified IKKalpha- and CD95- interacting proteins in primary human T cells, detected all major binders by mass spectrometry analysis and identified two novel CD95-associated proteins.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
  • Instrument: LTQ Orbitrap

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-06-12 06:09:52ID requested
12018-01-11 02:57:17announced

Publication List

  1. Sciuto MR, Warnken U, Schnölzer M, Valvo C, Brunetto L, Boe A, Biffoni M, Krammer PH, De Maria R, Haas TL, Two-Step Coimmunoprecipitation (TIP) Enables Efficient and Highly Selective Isolation of Native Protein Complexes. Mol Cell Proteomics, 17(5):993-1009(2018) [pubmed]

Keyword List

  1. curator keyword: Technical
  2. submitter keyword: Immunoprecipitation, co-immunoprecipitation, co-IP, TIP, bTIP, IKK, NFKB, CD95, CHIP, monoclonal, polyclonal, bridging, antibody, chromatin, primary cells

Contact List

    Martina Schnoelzer
    • contact affiliation: German Cancer Research Center (DKFZ), Heidelberg, Germany Functional Proteome Analysis
    • contact email: m.schnoelzer@dkfz.de
    • lab head:
    Uwe Warnken
    • contact affiliation: German Cancer Research Center (DKFZ)
    • contact email: U.Warnken@dkfz.de
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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