PXD006700 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Two-Step Co-Immunoprecipitation (TIP) Enables an Efficient and Highly Selective Isolation of Native Protein-Complexes |
Description | Co-immunoprecipitation (co-IP) is the most frequently used technique for the isolation of protein-protein (PPI) or protein-nucleic acid interactions (PNI) under native expression conditions. A major disadvantage of co-IP is the abundance of non-specific binders that can impede downstream applications. To overcome this limitation, we developed the two-step co-IP (TIP) that significantly improves the purification of native protein-containing complexes. TIP can be applied with a broad range of specific mono- and polyclonal antibodies. Using TIP we purified IKKalpha- and CD95- interacting proteins in primary human T cells, detected all major binders by mass spectrometry analysis and identified two novel CD95-associated proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2018-01-11 |
AnnouncementXML | Submission_2018-01-11_02:57:16.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Uwe Warnken |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-06-12 06:09:52 | ID requested | |
⏵ 1 | 2018-01-11 02:57:17 | announced | |
Publication List
Sciuto MR, Warnken U, Schn, รถ, lzer M, Valvo C, Brunetto L, Boe A, Biffoni M, Krammer PH, De Maria R, Haas TL, Two-Step Coimmunoprecipitation (TIP) Enables Efficient and Highly Selective Isolation of Native Protein Complexes. Mol Cell Proteomics, 17(5):993-1009(2018) [pubmed] |
Keyword List
curator keyword: Technical |
submitter keyword: Immunoprecipitation, co-immunoprecipitation, co-IP, TIP, bTIP, IKK, NFKB, CD95, CHIP, monoclonal, polyclonal, bridging, antibody, chromatin, primary cells |
Contact List
Martina Schnoelzer |
contact affiliation | German Cancer Research Center (DKFZ), Heidelberg, Germany Functional Proteome Analysis |
contact email | m.schnoelzer@dkfz.de |
lab head | |
Uwe Warnken |
contact affiliation | German Cancer Research Center (DKFZ) |
contact email | U.Warnken@dkfz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD006700 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD006700
- Label: PRIDE project
- Name: Two-Step Co-Immunoprecipitation (TIP) Enables an Efficient and Highly Selective Isolation of Native Protein-Complexes