PXD006690
PXD006690 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Molecular Determinants in Redox Homeostasis and NF-κB Signaling |
| Description | Mitochondrial protein (MP) dysfunction has been linked to neurodegenerative disorders (NDs), however, the discovery of molecular mechanisms underlying NDs has been impeded by the limited characterization of interactions governing MP function. Here, using 109 affinity-purified mitochondrial fractions isolated from 13 epitope-tagged human ND-linked MPs in HEK293 cells coupled with mass spectrometry (MS), we report a high-confidence MP network involving 1,964 interactions among 772 proteins (>90% previously unreported). Nearly half of these interactions were confirmed in differentiated neuronal cells by primary antibody immunoprecipitation and MS, with many linked to NDs and autism. We show that the SOD1-PRDX5 interaction, critical for mitochondrial redox homeostasis, can be perturbed by amyotrophic lateral sclerosis-linked variants, and establish a new functional role for ND-linked factors coupled with IκBε in NF-kB activation. Our results identify new mechanisms for MPs with direct consequences for human disease, and expands the interaction landscape of human mitochondria |
| HostingRepository | PRIDE |
| AnnounceDate | 2017-12-05 |
| AnnouncementXML | Submission_2017-12-05_09:26:04.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Sadhna Phanse |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | LTQ Orbitrap Elite |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2017-06-09 06:20:10 | ID requested | |
| ⏵ 1 | 2017-12-05 09:26:05 | announced |
Publication List
| Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson J, Babu M, B Signaling. Cell Syst, 5(6):564-577.e12(2017) [pubmed] |
Keyword List
| curator keyword: Biological, Biomedical |
| submitter keyword: Autism, Mitochondria, Mitochondrial Proteins, Neurodegeneration, NF-kB signaling, Parkinson's Disease, Protein-protein interactions, Proteomics |
Contact List
| Dr. Mohan Babu | |
|---|---|
| contact affiliation | Research and Innovation Centre, University of Regina, SK S4S OA2, Canada |
| contact email | mohan.babu@uregina.ca |
| lab head | |
| Sadhna Phanse | |
| contact affiliation | University of Toronto |
| contact email | srphanse@gmail.com |
| dataset submitter | |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD006690 |
| PRIDE project URI |
Repository Record List
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