PXD006686

PXD006686 is an original dataset announced via ProteomeXchange.

Title	Glycolytic Enzyme Coalesce in G bodies Under Hypoxic Stress
Description	Glycolysis is upregulated in cells under specific conditions, such as hypoxia and high energy demand, to achieve the metabolic requirements for continued cell proliferation. However, the mechanism of this increased glycolytic rate remains poorly understood. In the budding yeast Saccharomyces cerevisiae, we discovered that hypoxia induces concentration of many glycolytic enzymes, including the Pfk2p subunit of the ratelimiting enzyme, phosphofructokinase, into a single, non-membrane-bound granule that we define as the "glycolytic body" or "G body". Pfk2p localization to G bodies depends on its N-terminal, intrinsically disordered region. In order to identify factors important for G body formation, we conducted a yeast kinome screen and identified the AMP kinase ortholog, Snf1p, to be required for the localization of multiple glycolytic enzymes to G bodies. Further, proteomic analyses of purified G bodies identified a core set of resident factors, many of which are essential for G body integrity. Cells incapable of forming G bodies in hypoxic conditions display abnormal cell division and produce inviable daughter cells. Conversely, cells that form G bodies show increased glucose consumption and decreased levels of glycolytic intermediates. Importantly, G bodies also form in human, hepatocarcinoma cells upon hypoxic stress. Together, our results suggest that G body formation is a conserved, adaptive response to increase glycolytic output during hypoxia or tumorigenesis.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_12:59:58.392.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006686
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Kim
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-09 02:14:15	ID requested
⏵ 1	2021-03-04 12:59:58	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: hypoxia

glycolytic enzymes

RNA granules

AMP kinase

phosphorylation

Saccharomyces cerevisiae

phosphofructokinase

John K. Kim
contact affiliation	Department of Biology, Johns Hopkins University, Baltimore, MD 21218 USA
contact email	jnkim@jhu.edu
lab head
John Kim
contact affiliation	Johns Hopkins University
contact email	jnkim@jhu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006686
     1. Label: PRIDE project
     2. Name: Glycolytic Enzyme Coalesce in G bodies Under Hypoxic Stress