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PXD006679

PXD006679 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTreatment with Cefotaxime Affects Expression of Conjugation Associated Proteins and Conjugation Transfer Frequency of an IncI1 Plasmid in Escherichia coli
DescriptionHorizontal gene transfer (HGT) is the major mechanism responsible for spread of antibiotic resistance. Antibiotic treatment has been suggested to promote HGT, either by directly affecting the conjugation process itself or by selecting for conjugations subsequent to DNA transfer. However, recent research suggests that the effect of antibiotic treatment on plasmid conjugation frequencies, and hence the spread of resistance plasmids, may have been overestimated. We addressed the question by quantifying transfer proteins and conjugation frequencies of a blaCTX-M-1 encoding IncI1 resistance plasmid in Escherichia coli MG1655 in the presence and absence of therapeutically relevant concentrations of cefotaxime (CTX). Analysis of the proteome by iTRAQ labeling and liquid chromatography tandem mass spectrometry revealed that Tra proteins were significantly up regulated in the presence of CTX. The up-regulation of the transfer machinery was confirmed at the transcriptional level for five selected genes. The CTX treatment did not cause induction of the SOS39 response as revealed by absence of significantly regulated SOS associated proteins in the proteome and no significant up-regulation of recA and sfiA genes. The frequency of plasmid conjugation, measured in an antibiotic free environment, increased significantly when the donor was pre-grown in broth containing CTX compared to growth without this drug, regardless of whether blaCTX-M-1 was located on the plasmid or in trans on the chromosome. The results shows that antibiotic treatment can affect expression of a plasmid conjugation machinery and subsequent DNA transfer.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:38:35.120.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnders Boysen
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationListiTRAQ4plex-116 reporter+balance reagent acylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-06-08 03:12:16ID requested
12017-11-21 06:06:29announced
22018-05-23 06:28:52announcedUpdated publication reference for PubMed record(s): 29238335.
32024-10-22 04:38:43announced2024-10-22: Updated project metadata.
Publication List
10.3389/fmicb.2017.02365;
M, ø, ller TSB, Liu G, Boysen A, Thomsen LE, L, ü, thje FL, Mortensen S, M, ø, ller-Jensen J, Olsen JE, . Front Microbiol, 8():2365(2017) [pubmed]
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: antibiotics, proteomics,Escherichia coli, iTRAQ
Contact List
Jakob Møller-Jensen
contact affiliationDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark
contact emailJakobm@bmb.sdu.dk
lab head
Anders Boysen
contact affiliationUniversity of Southern Denmark
contact emailBoysen@bmb.sdu.dk
dataset submitter
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Dataset FTP location
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PRIDE project URI
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