PXD006679

PXD006679 is an original dataset announced via ProteomeXchange.

Title	Treatment with Cefotaxime Affects Expression of Conjugation Associated Proteins and Conjugation Transfer Frequency of an IncI1 Plasmid in Escherichia coli
Description	Horizontal gene transfer (HGT) is the major mechanism responsible for spread of antibiotic resistance. Antibiotic treatment has been suggested to promote HGT, either by directly affecting the conjugation process itself or by selecting for conjugations subsequent to DNA transfer. However, recent research suggests that the effect of antibiotic treatment on plasmid conjugation frequencies, and hence the spread of resistance plasmids, may have been overestimated. We addressed the question by quantifying transfer proteins and conjugation frequencies of a blaCTX-M-1 encoding IncI1 resistance plasmid in Escherichia coli MG1655 in the presence and absence of therapeutically relevant concentrations of cefotaxime (CTX). Analysis of the proteome by iTRAQ labeling and liquid chromatography tandem mass spectrometry revealed that Tra proteins were significantly up regulated in the presence of CTX. The up-regulation of the transfer machinery was confirmed at the transcriptional level for five selected genes. The CTX treatment did not cause induction of the SOS39 response as revealed by absence of significantly regulated SOS associated proteins in the proteome and no significant up-regulation of recA and sfiA genes. The frequency of plasmid conjugation, measured in an antibiotic free environment, increased significantly when the donor was pre-grown in broth containing CTX compared to growth without this drug, regardless of whether blaCTX-M-1 was located on the plasmid or in trans on the chromosome. The results shows that antibiotic treatment can affect expression of a plasmid conjugation machinery and subsequent DNA transfer.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:38:35.120.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anders Boysen
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-08 03:12:16	ID requested
1	2017-11-21 06:06:29	announced
2	2018-05-23 06:28:52	announced	Updated publication reference for PubMed record(s): 29238335.
⏵ 3	2024-10-22 04:38:43	announced	2024-10-22: Updated project metadata.

10.3389/fmicb.2017.02365;

M, ø, ller TSB, Liu G, Boysen A, Thomsen LE, L, ü, thje FL, Mortensen S, M, ø, ller-Jensen J, Olsen JE, . Front Microbiol, 8():2365(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: antibiotics, proteomics,Escherichia coli, iTRAQ

Jakob Møller-Jensen
contact affiliation	Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark
contact email	Jakobm@bmb.sdu.dk
lab head
Anders Boysen
contact affiliation	University of Southern Denmark
contact email	Boysen@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD006679

PRIDE project URI

• PRIDE
  1. PXD006679
     1. Label: PRIDE project
     2. Name: Treatment with Cefotaxime Affects Expression of Conjugation Associated Proteins and Conjugation Transfer Frequency of an IncI1 Plasmid in Escherichia coli