PXD006676

PXD006676 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterisation of Caenorhabditis elegans larval development
Description	The nematode Caenorhabditis elegans (C. elegans) is often used as a model organism to study cell and developmental biology. Quantitative mass spectrometry has only recently been performed in C. elegans and, so far, most studies have been done on adult worm samples. Here we use quantitative mass spectrometry to characterise protein level changes across the four larval developmental stages (L1-L4) of C. elegans, in biological triplicate. In total, we identify 4,130 proteins and quantify 1,541 proteins that were identified across all four stages in all three biological repeats with at least 2 unique peptides per protein. Using hierarchical clustering and functional ontological analyses, we identify 21 protein groups containing proteins with similar protein profiles across the four stages, and highlight the most overrepresented biological functions in each of these protein clusters. In addition, we use the dataset to identify putative larval stage specific proteins in each individual developmental stage, as well as in the early and late developmental stages. In summary, this dataset provides a system-wide analysis of protein level changes across the four C. elegans larval developmental stages, which serves as a useful resource for the worm development research community.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:27:39.040.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thomas Cox
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-08 01:32:40	ID requested
1	2017-12-06 07:49:51	announced
⏵ 2	2024-10-22 04:27:47	announced	2024-10-22: Updated project metadata.

Xia T, Horton ER, Salcini AE, Pocock R, Cox TR, Erler JT, Proteomic Characterization of Caenorhabditis elegans Larval Development. Proteomics, 18(2):(2018) [pubmed]

10.1002/pmic.201700238;

curator keyword: Biological

submitter keyword: Caenorhabditis elegans, LC-MS, Larvae, Development

Janine T Erler
contact affiliation	Biotech Research and Innovation Centre (BRIC), University of Copenhagen (UCPH), Ole Maaløes Vej 5, 2200 Copenhagen, Denmark
contact email	janine.erler@bric.ku.dk
lab head
Thomas Cox
contact affiliation	Garvan Institute of Medical Research, The Kinghorn Cancer Centre, Sydney, Australia
contact email	t.cox@garvan.org.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD006676

PRIDE project URI

• PRIDE
  1. PXD006676
     1. Label: PRIDE project
     2. Name: Proteomic characterisation of Caenorhabditis elegans larval development