PXD006674

PXD006674 is an original dataset announced via ProteomeXchange.

Title	Comparative degradomics of porcine and human wound exudates unravels biomarker candidates for assessment of wound healing progression in trauma patients
Description	Impaired cutaneous wound healing is a major complication in elderly people and patients suffering from diabetes with raising rates in industrialized countries. Heterogeneity of clinical manifestations hampers effective molecular diagnostics and decisions for appropriate therapeutic regimens. Using a customized positional quantitative proteomics workflow, we have established a time-resolved proteome and N-terminome resource from wound exudates in a clinical pig wound model that we exploited as robust template to interpret a heterogeneous dataset from patients undergoing the same wound treatment. With Zyxin, IQGAP1 and HtrA1, this analysis and validation by targeted proteomics identified differential abundances and proteolytic processing of proteins of epidermal and dermal origin as prospective biomarker candidates for assessment of critical turning points in wound progression. Thus, we demonstrate the power of a fine-tuned animal wound model to bridge the translational gap as prerequisite for future extended clinical studies with large cohorts of individuals affected by healing impairments.
HostingRepository	PRIDE
AnnounceDate	2017-09-14
AnnouncementXML	Submission_2017-09-14_06:28:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ulrich auf dem Keller
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-08 01:25:39	ID requested
⏵ 1	2017-09-14 06:28:09	announced

Sabino F, Egli FE, Savickas S, Holstein J, Kaspar D, Rollmann M, Kizhakkedathu JN, Pohlemann T, Smola H, Auf dem Keller U, Patients. J Invest Dermatol, 138(2):413-422(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: impaired wound healing, negative pressure wound therapy, wound fluids, positional proteomics, TAILS, biomarker

Ulrich auf dem Keller
contact affiliation	ETH Zurich, Department of Biology, Institute of Molecular Health Sciences
contact email	ulrich.aufdemkeller@biol.ethz.ch
lab head
Ulrich auf dem Keller
contact affiliation	Department of Biology
contact email	ulrich.aufdemkeller@biol.ethz.ch
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006674

PRIDE project URI

• PRIDE
  1. PXD006674
     1. Label: PRIDE project
     2. Name: Comparative degradomics of porcine and human wound exudates unravels biomarker candidates for assessment of wound healing progression in trauma patients