PXD006672

PXD006672 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis reveals cytoskeletal remodelling via CDC42-PAK signaling in lung mucoepidermoid cells upon chronic exposure to cigarette
Description	Despite the fact that smoking is the major cause of lung cancer, there is no clear mechanism detailing how chronic exposure of cigarette smoke alters the global signaling in lung cells. To investigate the altered signaling in lung cells, in this study, we carried out SILAC-based quantitative phosphoproteomic analysis of H292 cells chronically exposed to cigarette smoke. Using high resolution Orbitrap Velos mass spectrometer, we identified hyperphosphorylation of 493 sites which corresponds to 341 proteins and 195 hypophosphorylated sites mapping to 142 proteins, upon smoke exposure (2.0-fold). Bioinformatics analysis revealed differentially phosphotylated proteins were involved in multiple cellular processes including cellular polarity, cytoskeletal remodelling, cellular migration, protein synthesis, autophagy and apoptosis. The present study will significantly improve the current knowledge of smoke mediated signaling in lung cells, which in turn may aid in the development of targeted therapies specially for smokers.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:38:13.903.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aditi Chatterjee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; 6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-08 01:15:53	ID requested
1	2018-10-24 12:41:27	announced
⏵ 2	2024-10-22 04:38:22	announced	2024-10-22: Updated project metadata.

Solanki HS, Advani J, Khan AA, Radhakrishnan A, Sahasrabuddhe NA, Pinto SM, Chang X, Prasad TSK, Mathur PP, Sidransky D, Gowda H, Chatterjee A, Chronic Cigarette Smoke Mediated Global Changes in Lung Mucoepidermoid Cells: A Phosphoproteomic Analysis. OMICS, 21(8):474-487(2017) [pubmed]

10.1089/omi.2017.0090;

curator keyword: Biomedical

submitter keyword: NSCLC, Smoking, In vivo labelling, phosphoproteomics

Aditi Chatterjee
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India
contact email	aditixchatterjee@gmail.com
lab head
Aditi Chatterjee
contact affiliation	Institute of Bioinformatics, Bangalore, Karnataka, India
contact email	aditixchatterjee@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD006672

PRIDE project URI

• PRIDE
  1. PXD006672
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis reveals cytoskeletal remodelling via CDC42-PAK signaling in lung mucoepidermoid cells upon chronic exposure to cigarette