PXD006665

PXD006665 is an original dataset announced via ProteomeXchange.

Title	A Proteomic Survey of Enzymes in the Plasma and Gastric Fluid of Burmese Python
Description	Background: Exceptional and extreme feeding behaviour makes the Burmese python (Python bivittatus) an interesting model to study physiological remodelling and metabolic adaptation in response to refeeding after prolonged starvation. In this study, we used transcriptome sequencing of five visceral organs during fasting as well as 24h and 48h after ingestion of a large meal to unravel the postprandial changes in Burmese pythons. We first used the pooled data to perform a de novo assembly of the transcriptome and supplemented this with a proteomic survey of enzymes in the plasma and gastric fluid. Results: We constructed a high-quality transcriptome with 34,423 transcripts of which 19,713 (57%) were annotated. Among highly expressed genes (FPKM>100 in one tissue) we found the transition from fasting to digestion was associated with differential expression of 43 genes in the heart, 206 genes in the liver, 114 genes in the stomach, 89 genes in the pancreas and 158 genes in the intestine. We interrogated the function of these genes to test previous hypotheses on the response to feeding. We also used the transcriptome to identify 314 secreted proteins in the gastric fluid of the python Conclusions: Digestion was associated with an upregulation of genes related to metabolic processes, and translational changes therefore appears to support the postprandial rise in metabolism. We identify stomach-related proteins from a digesting individual and demonstrate that the sensitivity of modern LC-MS/MS equipment allows the identification of gastric juice proteins that are present during digestion.
HostingRepository	PRIDE
AnnounceDate	2018-10-24
AnnouncementXML	Submission_2018-10-24_12:42:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinjie Duan
SpeciesList	scientific name: Python bivittatus; NCBI TaxID: 176946;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-06 08:04:39	ID requested
⏵ 1	2018-10-24 12:42:41	announced

Duan J, Sanggaard KW, Schauser L, Lauridsen SE, Enghild JJ, Schierup MH, Wang T, Transcriptome analysis of the response of Burmese python to digestion. Gigascience, 6(8):1-18(2017) [pubmed]

curator keyword: Biological

submitter keyword: Burmese Python, Plasma, Gastric Fluid, LC-MS/MS

Tobias Wang
contact affiliation	Professor, Department of Bioscience, Aarhus University, Denmark
contact email	tobias.wang@bios.au.dk
lab head
Jinjie Duan
contact affiliation	Aarhus University
contact email	jjduan.birc@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006665
     1. Label: PRIDE project
     2. Name: A Proteomic Survey of Enzymes in the Plasma and Gastric Fluid of Burmese Python