PXD006660

PXD006660 is an original dataset announced via ProteomeXchange.

Title	Capturing protein communities by structural proteomics in a thermophilic eukaryote
Description	The arrangement of proteins into complexes is a key organizational principle for many cellular functions. Although the topology of many complexes has been systematically analyzed in isolation, their molecular sociology in situ remains elusive. Here, we show that crude cellular extracts of a eukaryotic thermophile, Chaetomium thermophilum, retain basic principles of cellular organization. Using a structural proteomics approach, we simultaneously characterized the abundance, interactions and structure of a third of the C. thermophilum proteome within these extracts. We identified 27 distinct protein communities that include 108 interconnected complexes, which dynamically associate with each other and functionally benefit from being in close proximity in the cell.
HostingRepository	PRIDE
AnnounceDate	2017-08-07
AnnouncementXML	Submission_2017-08-07_04:21:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katarzyna Buczak
SpeciesList	scientific name: Chaetomium thermophilum; NCBI TaxID: 209285;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-06 04:50:29	ID requested
⏵ 1	2017-08-07 04:21:52	announced

Kastritis PL, O'Reilly FJ, Bock T, Li Y, Rogon MZ, Buczak K, Romanov N, Betts MJ, Bui KH, Hagen WJ, Hennrich ML, Mackmull MT, Rappsilber J, Russell RB, Bork P, Beck M, Gavin AC, Capturing protein communities by structural proteomics in a thermophilic eukaryote. Mol Syst Biol, 13(7):936(2017) [pubmed]

curator keyword: Biological

submitter keyword: XL-MS, Chaetomium thermophilum, protein communities,

Martin Beck
contact affiliation	European Molecular Biology Laboratory, Structural and Computational Biology Unit, Heidelberg, Germany
contact email	martin.beck@embl.de
lab head
Katarzyna Buczak
contact affiliation	European Molecular Biology Laboratory
contact email	buczak@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD006660

PRIDE project URI

• PRIDE
  1. PXD006660
     1. Label: PRIDE project
     2. Name: Capturing protein communities by structural proteomics in a thermophilic eukaryote