PXD006657

PXD006657 is an original dataset announced via ProteomeXchange.

Title	Defining the hydrogen peroxide-dependent redoxome in human cells
Description	Protein cysteinyl residues are the mediators of hydrogen peroxide (H2O2)-dependent redox signaling. However, site-specific mapping of the selectivity and dynamics of these redox reactions in cells poses a major analytical challenge. Here we describe a chemoproteomic platform to systematically and quantitatively analyze the reactivity of thousands of cysteines toward H2O2 in human cells. We identified >900 H2O2-sensitive cysteines, which are defined as the H2O2-dependent redoxome. Although redox sites associated with antioxidative and metabolic functions are consistent, most of the H2O2-dependent redoxome varies dramatically between different cells. Structural analyses reveal that H2O2-sensitive cysteines are less conserved than their redox-insensitive counterparts and display distinct sequence motifs, structural features, and potential for crosstalk with lysine modifications. Notably, our chemoproteomic platform also provides an opportunity to predict oxidation-triggered protein conformational changes. The data are freely accessible as a resource at http://redox.ncpsb.org/OXID/.
HostingRepository	PRIDE
AnnounceDate	2021-02-18
AnnouncementXML	Submission_2021-02-17_23:07:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jing Yang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-05 08:35:25	ID requested
⏵ 1	2021-02-17 23:07:35	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Chemoproteomics

Jing Yang
contact affiliation	National Center for Protein Science Beijing
contact email	yangjing54@hotmail.com
lab head
Jing Yang
contact affiliation	National Center for Protein Sciences, Beijing
contact email	yangjing54@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD006657
     1. Label: PRIDE project
     2. Name: Defining the hydrogen peroxide-dependent redoxome in human cells