PXD006656

PXD006656 is an original dataset announced via ProteomeXchange.

Title	Mapping the spatial proteome of metastatic cells in colorectal cancer
Description	Here, we aimed to study metastasis mechanisms using spatial proteomics applied to the KM12 cell model of metastasis. KM12 cells were metabolically labelled using SILAC. SILAC has been successfully used for the analysis of proteome turnover and for the identification of changes in proteome localization as a consequence of DNA damage.10,23-24 Subcellular fractionation of the KM12 cells into five subcellular fractions corresponding to cytoplasm (CEB), plasma, mitochondria and ER/golgi membranes (MEB), nuclear (NEB), chromatin-bound (NEB-CBP) and cytoskeletal proteins (PEB) contributed to clarify the molecular mechanisms underlying CRC metastasis. Protein abundance and localization were measured in parallel for the five separate subcellular fractions, providing a map draft of the spatially deregulated protein complexes and networks in CRC metastasis.
HostingRepository	PRIDE
AnnounceDate	2017-09-11
AnnouncementXML	Submission_2017-09-11_06:08:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alberto Pelaez García
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-05 03:28:12	ID requested
⏵ 1	2017-09-11 06:08:41	announced

Mendes M, Pel, á, ez-Garc, í, a A, L, ó, pez-Lucendo M, Bartolom, é RA, Calvi, ñ, o E, Barderas R, Casal JI, Mapping the Spatial Proteome of Metastatic Cells in Colorectal Cancer. Proteomics, 17(19):(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Colorectal cancer, SILAC, Subcellular fraction, LC-MS/MS

Ignacio Casal
contact affiliation	Functional Proteomics Laboratory, Centro de Investigaciones Biologicas, CIB-CSIC, Madrid, Spain
contact email	albertopelaez84@gmail.com
lab head
Alberto Pelaez García
contact affiliation	Hospital Univ. La Paz. Molecular Pathology Lab.
contact email	albertopelaez84@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006656

PRIDE project URI

• PRIDE
  1. PXD006656
     1. Label: PRIDE project
     2. Name: Mapping the spatial proteome of metastatic cells in colorectal cancer