PXD006650

PXD006650 is an original dataset announced via ProteomeXchange.

Title	Human, melanoma, p62/SQSTM1, quantitative proteomics
Description	p62/SQSTM1 was identified as a modulator of metastatic genes selectively enriched in melanoma in autophagy independent manner. iTRAQ quantitative proteomic approach was performed in melanoma cell lines (SK-Mel-103 and UACC-62) deficient for p62 to identify downstream effectors of p62. Similar studies were performed for ATG5, a core component of autophagy, as a reference for autophagy-associated changes in protein abundance. Additionally, melanoma cells were subjected to affinity purification (AP-MS) to identify the interactors of p62. Overall, these studies underscore a novel unexpected role of p62 regulating the stability of prometastatic factors via the interaction with RNA Binding Proteins, thus leading to the inhibition of protein translation.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:01.424.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pilar Ximenez-Embun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iTRAQ4plex-116 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-02 08:20:20	ID requested
1	2022-02-28 02:46:33	announced
⏵ 2	2024-10-22 05:34:02	announced	2024-10-22: Updated project metadata.

Karras P, Riveiro-Falkenbach E, Ca, ñ, ó, n E, Tejedo C, Calvo TG, Mart, í, nez-Herranz R, Alonso-Curbelo D, Cifdaloz M, Perez-Guijarro E, G, ó, mez-L, ó, pez G, Ximenez-Embun P, Mu, ñ, oz J, Megias D, Olmeda D, Moscat J, Ortiz-Romero PL, Rodr, í, guez-Peralto JL, Soengas MS, p62/SQSTM1 Fuels Melanoma Progression by Opposing mRNA Decay of a Selective Set of Pro-metastatic Factors. Cancer Cell, 35(1):46-63.e10(2019) [pubmed]

10.1016/j.ccell.2018.11.008;

curator keyword: Biomedical

submitter keyword: Human, AP-MS, p62/SQSTM1, melanoma cell lines, quantitative proteomics, LC-MS/MS

Javier Muñoz Peralta
contact affiliation	Proteomics Unit Spanish National Cancer Research Centre (CNIO) Madrid,SPAIN
contact email	jmunozpe@cnio.es
lab head
Pilar Ximenez-Embun
contact affiliation	Proteomics Group
contact email	mpximenez@cnio.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD006650

PRIDE project URI

• PRIDE
  1. PXD006650
     1. Label: PRIDE project
     2. Name: Human, melanoma, p62/SQSTM1, quantitative proteomics