PXD006645

PXD006645 is an original dataset announced via ProteomeXchange.

Title	Glareosin: a novel sexually dimorphic urinary lipocalin in the bank vole, Myodes glareolus, C4PR_LIV
Description	The urine of bank voles (Myodes glareolus) contains substantial quantities of a small protein that is expressed at much higher levels in males than females, and at higher levels in males in the breeding season. This protein was purified and completely sequenced at the protein level by mass spectrometry. Leucine/isoleucine ambiguity was completely resolved by metabolic labelling, monitoring the incorporation of dietary deuterated leucine into specific sites in the protein. The predicted mass of the sequenced protein was exactly consonant with the mass of the protein measured in bank vole urine samples, correcting for the formation of two disulphide bonds. The sequence of the protein revealed that it was a lipocalin related to aphrodisin and other odorant binding proteins (OBPs), but differed from all OBPs previously described. The pattern of secretion in urine used for scent marking by male bank voles, and similarity to other lipocalins used as chemical signals in rodents, suggest that this protein plays a role in male sexual and/or competitive communication. We propose the name glareosin for this novel protein to reflect the origin of the protein and to emphasise the distinction from known OBPs.
HostingRepository	PRIDE
AnnounceDate	2017-09-13
AnnouncementXML	Submission_2017-09-13_00:06:42.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD006645
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Grace Loxley
SpeciesList	scientific name: Myodes glareolus (Bank vole) (Clethrionomys glareolus); NCBI TaxID: 447135;
ModificationList	Label:2H(3); Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-06-01 04:58:23	ID requested
⏵ 1	2017-09-13 00:06:43	announced

Loxley GM, Unsworth J, Turton MJ, Jebb A, Lilley KS, Simpson DM, Rigden DJ, Hurst JL, Beynon RJ, . Open Biol, 7(9):(2017) [pubmed]

curator keyword: Biological

submitter keyword: Lipocalin, bank vole, Myodes glareoulus, odorant binding protein, OBP, metabolic labelling, mass spectrometry, primary sequence, glareosin

Robert Beynon
contact affiliation	Centre for Proteome Research, University of Liverpool
contact email	r.beynon@liverpool.ac.uk
lab head
Grace Loxley
contact affiliation	University of Liverpool
contact email	G.M.Loxley@liverpool.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD006645

PRIDE project URI

• PRIDE
  1. PXD006645
     1. Label: PRIDE project
     2. Name: Glareosin: a novel sexually dimorphic urinary lipocalin in the bank vole, Myodes glareolus, C4PR_LIV