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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-09-13
  • AnnouncementXML: Submission_2017-09-13_00:06:42.xml
  • DigitalObjectIdentifier: http://dx.doi.org/10.6019/PXD006645
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Supported dataset by repository
  • PrimarySubmitter: Grace Loxley
  • Title: Glareosin: a novel sexually dimorphic urinary lipocalin in the bank vole, Myodes glareolus, C4PR_LIV
  • Description: The urine of bank voles (Myodes glareolus) contains substantial quantities of a small protein that is expressed at much higher levels in males than females, and at higher levels in males in the breeding season. This protein was purified and completely sequenced at the protein level by mass spectrometry. Leucine/isoleucine ambiguity was completely resolved by metabolic labelling, monitoring the incorporation of dietary deuterated leucine into specific sites in the protein. The predicted mass of the sequenced protein was exactly consonant with the mass of the protein measured in bank vole urine samples, correcting for the formation of two disulphide bonds. The sequence of the protein revealed that it was a lipocalin related to aphrodisin and other odorant binding proteins (OBPs), but differed from all OBPs previously described. The pattern of secretion in urine used for scent marking by male bank voles, and similarity to other lipocalins used as chemical signals in rodents, suggest that this protein plays a role in male sexual and/or competitive communication. We propose the name glareosin for this novel protein to reflect the origin of the protein and to emphasise the distinction from known OBPs.
  • SpeciesList: scientific name: Myodes glareolus (Bank vole) (Clethrionomys glareolus); NCBI TaxID: 447135;
  • ModificationList: Label:2H(3); Oxidation; Carbamidomethyl
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-06-01 04:58:23ID requested
12017-09-13 00:06:43announced

Publication List

  1. Loxley GM, Unsworth J, Turton MJ, Jebb A, Lilley KS, Simpson DM, Rigden DJ, Hurst JL, Beynon RJ, . Open Biol, 7(9):(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Lipocalin, bank vole, Myodes glareoulus, odorant binding protein, OBP, metabolic labelling, mass spectrometry, primary sequence, glareosin

Contact List

    Robert Beynon
    • contact affiliation: Centre for Proteome Research, University of Liverpool
    • contact email: r.beynon@liverpool.ac.uk
    • lab head:
    Grace Loxley
    • contact affiliation: University of Liverpool
    • contact email: G.M.Loxley@liverpool.ac.uk
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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